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- This enables the analysis of liquids, biological material and "wet" food products to be analysed in the SEM.
- This is done by first plunging the sample into nitrogen slush and the transferring the sample into the cryo-chamber.
- Subequently the sample is allowed to warm, thus allowing the ice to shrink and reveal the sample surface. Fracturing can also be performed at this point if needed.
- The sample will then be sputter coated in platinum to become conductive and transferred into the SEM chamber, where it remains frozen on another cold-stage.
- The minimum operating temperature for this technique is -140°.