- A406, Portland Square, Drake Circus, Plymouth, Devon, PL4 8AA
- +44 1752 584888
- daniel.merrifield@plymouth.ac.uk
Profiles
Dr Daniel Merrifield
Associate Professor of Fish Health and Nutrition
School of Biological & Marine Sciences (Faculty of Science & Engineering)
Role
Research and teaching
Qualifications
PhD - Evaluation of selected probiotics and bovine lactoferrin asfeed supplements for rainbow trout (Oncorhynchus mykiss Walbaum) forapplications in aquaculture. Plymouth University
Postgraduate Certificate in Academic Practice (PGCAP), Plymouth University
PgCert (distinction) -Research Methods, Plymouth University
General Teachers Associates training course, Plymouth University
BSc (Hons; 2:1) - BiologicalSciences, Plymouth University
Professional membership
Society for Applied Microbiology
World Aquaculture Society Publication Committee
Fellow of the Higher Education Academy (FHEA)
Environment, Food and Biotechnology Research Group
Roles on external bodies
Editor-in-Chief for the Journal ofAquaculture Research and Development (2010-2014)
Chief Editor of the Fisheries and Aquaculture Journal (2011-2013)
Chief Editor for specialissue on probiotics and prebiotics in aquaculture, Journal ofAquaculture Research and Development (2011)
Associate Editor for the Journalof the World Aquaculture Society (2015-present)
Member of the Editorial board for
Ø PLoSONE (2012-present)
Ø Aquaculture Research (2010-present),
Ø Aquaculture International (2011-present)
Ø International Aquafeed(2010-present)
Member of the Book Publication Committee for the World Aquaculture Society (2011-present)
Referee for the following scientific research bodies:
The Biotechnology and Biological Sciences Research Council (BBSRC),UK
The United States Department of Agriculture (USDA)
The French National Research Agency(Agence Nationale de la Recherche;ANR)
The Portuguese Foundation for Science and Technology (FCT)
The Netherlands Organisation for Scientific Research (NWO)
Superior Council of the National Fund for Scientific & Technological Development (FONDECYT), Chile
National Research Foundation (NRF), South Africa
The National Science Centre (NCN), Poland
The National Agency for the Evaluation of Universities and Research Institutes (ANVUR), Italy;eValuation of Quality of Research (VQR)2004-2010
Wageningen Institute of Animal Science (WIAS), Netherlands
IdEx Bordeaux Post-doctoral program, University of Bordeaux
Referee for numerous journals, including:
African Journal of Biotechnology
AJP - Regulatory, Integrative and Comparative Physiology
Annals of Microbiology
Antonie van Leeuwenhoek Journal of Microbiology
Archives of Microbiology
Aquaculture
Aquaculture International
Aquaculture Nutrition
Aquaculture Research
British Journal of Nutrition
Chemistry and Ecology
FEMS Microbial Ecology
Fish and Fisheries
Fish Physiology and Biochemistry
Fish and Shellfish Immunology
Genetic Engineering and Biotechnology Journal
International Research Journal of Microbiology
Journal of Animal Sciences
Journal of Aquatic animal Health
Journal of Fish Biology
Journal of Fish Diseases
Journal of the World Aquaculture Society
Microbial Cell Factories
Public Library of Science ONE (PLoS ONE
Teaching interests
Topics:
Gut health and microecology
Aquatic microbiology
Aquaculture
Probiotics and prebiotics
Programme Leader:
MSc(PgDip) Sustainable Aquaculture Systems
Module leader:
BIOL120 Cell Biology and Microbiology
BIO5208 Contemporary Issues in Aquaculture
Teaching on modules:
BIO504: Health and Production in Aquaculture
BIO504: Health and Production in Aquaculture
BIO505: Research Project
BIO5125: Sustainable Use of Resources in Biological Systems
BIOL119: Introduction to Biology
BIOL120: Cell Biology and Microbiology
BIOL121: Animal Physiology and Microbiology
BIOL123: Field Biology
BIOL126: Animal Behaviour Field Biology
MBIO322: Marine Microbiology - Ecology and Applications
BIOL5129: Health and Production in Aquaculture
BIO5131: Postgraduate Research Skills & Methods
Courses:
MSc(PgDip) Sustainable Aquaculture Systems
BSc(Hons) Biological Sciences
BSc(Hons) Animal Behaviour and Welfare
Research interests
Feed additives
Gut health
Alternative protein sources
Probiotics and Prebiotics
Research degrees awarded to supervised students
Dr Ana Rodiles - Post-Doctoral Research Associate
Dr Mark Rawling - Post-Doctoral Research Associate
HugoAlexander Jaramillo Torres - PhD student; Probiotics in aquaculture
SamVoller - PhD student; Alternative protein sources and prebiotics in salmonidaquafeeds
RungtawanYomla - PhD student; Development of autochthonous probiotics for applicationsfor tilapia
Gabriella Do Vale Pereira - PhD student; Development of probiotics for thesustainable cultivation of pirarucu, Arapaima giga
Kurt Servin - PhD student; The use of yeast-derived feed additives to support in shrimp health and production (part-time, overseas)
Nicola Pontefract – PhD student; Feed additive strategies to improve mucosal defensesin fish
Grants & contracts
Fundinggenerated: >£1,000,000 (RCUK= £320,000, other FEC projects = >£305,000, studentships = >£375,000,other = <£13,500)
Examples include (past 5 years):
| £325,000 (PU: £125,000) | BBSRC project (BB/N024028/1): Mucosal health and microbiota during sea lice parasitism: effect of oral delivery of immunomodulants, 2016-2018 (PI) salmonlousemicrobiome.org |
| £7,850 | Contractual work, Lallemand SAS, 2015-2016 (PI) |
| £8,000 | Leiber GmbH, contractual work, 2014 (PI) |
| £300,000 (PU: £60,000) | BBSRC-NERC project (BB/M026604/1): Gut health and immune function: the emerging role of gut microbiota in sustainable aquaculture, 2015-2017 (Co-I) |
| £5,697 | Lallemand, commissioned work, 2015 (PI) |
| £12,400 | British Council; Newton Bhabha Fund Grant, 2015 |
| £75,000 | Postdoctoral Fellowship, Lallemand Inc. 2014-2016 (PI) |
| £10,723 | Lallemand, commissioned work, 2015 (PI) |
| £9,600 | Contractual work, Oslo Veterinary School 2014 (PI) |
| £3,470 | Leiber GmbH, contractual work, 2014 (PI) |
| £10,300 | Tereos-Syral, contractual work, 2014 (PI) |
| £6,700 | Gut health and Probiotic workshop, CENIACUA, Colombia 2014 (PI) |
| £38,500 | Alltech contractual work, 2012-2015 (PI) |
| £23,500 | Lallemand Inc., commissioned work 2013-2014 (PI) |
| £12,800 | Plymouth Marine Laboratories commissioned work, 2012-2013 (PI) |
| €60,000 | PhD studentship, Tereos Syral, 2012-2016 (Sam Voller) (DoS) |
| £10,774 | Lallemand Inc commissioned work, 2012 (PI) |
| £10,650 | Seale-Hayne Educational Trust, 2012-2013 (PI) |
| £15,000 | ABAgri, commissioned work, 2012 (PI) |
| $48,500 | Novus International, ResM studentship 2011-2013 (David Peggs) (DoS) |
| £62,000 | Biomin-FST PU PhD studentship (50% industry/50% PU) 2011-2014 (Benedict Standen) (DoS) |
| £12,500 | IRNC-Lallemand Inc. research project, 2011-2012 (PI) |
| $7,500 | Novus International, commissioned work, 2011-2012 (PI) |
| €10,000 | Lallemand Inc, commissioned work, 2011 (PI) |
| £9,895 | University of Plymouth’s International Research Networking and Collaboration (IRNC) Scheme, 2010 (PI) |
Research groups
- Food, Nutrition and Health
Key publications are highlighted
Journals
Articles
2017
'Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish'
Scientific Reports
7,
(1)
, DOI
2017
'Effect of fishmeal and fish oil replacement by vegetable meals and oils on gut health of European sea bass (Dicentrarchus labrax)'
Aquaculture
468,
386-398
, DOI
2017
'In vitro selection of a synbiotic and in vivo evaluation on intestinal microbiota, performance and physiological response of rainbow trout ( Oncorhynchus mykiss ) fingerlings'
Aquaculture Nutrition
23,
(1)
111-118
, DOI
2016
'A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research'
Scientific Reports
, DOI
PEARL
2016
'Alternative protein sources in the diet modulate microbiota and functionality in the distal intestine of Atlantic salmon ( Salmo salar )'
Applied and Environmental Microbiology
AEM.02615-16-AEM.02615-16
, DOI
PEARL
2016
'Combined effects of exogenous enzymes and probiotic on Nile tilapia (Oreochromis niloticus) growth, intestinal morphology and microbiome'
Aquaculture
PEARL
2016
'Dietary administration of a commercial mixed-species probiotic improves growth performance and modulates the intestinal immunity of tilapia, Oreochromis niloticus'
Fish & Shellfish Immunology
49,
427-435
, DOI
PEARL
2016
'Effect of dietary components on the gut microbiota ofaquatic animals. A never-ending story?'
AQUACULTURE NUTRITION
22,
(2)
219-282
, DOI
2016
'Effects of dietary Nutrafito Plus on growth, haemotological parameters and total ammonia-nitrogen excretion of juvenile striped catfish Pangasianodon hypophthalmus'
Aquaculture Research
47,
(6)
1770-1777
, DOI
2016
'Effects of rearing temperature and dietary short-chain fructooligosaccharides supplementation on allochthonous gut microbiota, digestive enzymes activities and intestine health of turbot ( Scophthalmus maximus L.) juveniles'
Aquaculture Nutrition
22,
(3)
631-642
, DOI
2016
'Hydrolysed wheat gluten as part of a diet based on animal and plant proteins supports good growth performance of Asian seabass (Lates calcarifer), without impairing intestinal morphology or microbiota'
Aquaculture
453,
40-48
, DOI
2016
'Probiotic treatment reduces appetite and glucose level in the zebrafish model'
Scientific Reports
6,
(1)
, DOI
2016
'Supplementation of formulated diets for tilapia (Oreochromis niloticus) with selected exogenous enzymes: Overall performance and effects on intestinal histology and microbiota'
Animal Feed Science and Technology
215,
133-143
Publisher Site
, DOI
PEARL
2015
'Effects of short-chain fructooligosaccharides on growth performance and hepatic intermediary metabolism in turbot ( Scophthalmus maximus ) reared at winter and summer temperatures'
Aquaculture Nutrition
21,
(4)
433-443
, DOI
2015
'First report on the autochthonous gut microbiota of brown trout ( Salmo trutta Linnaeus)'
Aquaculture Research
46,
(12)
2962-2971
, DOI
2015
'Lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism'
Sci Rep
5,
, DOI
PEARL
2015
'Modulation of the intestinal microbiota and morphology of tilapia, Oreochromis niloticus, following the application of a multi-species probiotic'
Applied Microbiology and Biotechnology
99,
(20)
8403-8417
, DOI
PEARL
2015
'The autochthonous microbiota of the freshwater omnivores jundia (Rhamdia quelen) and tilapia (Oreochromis niloticus) and the effect of dietary carbohydrates'
AQUACULTURE RESEARCH
46,
(2)
472-481
, DOI
2014
'Dietary modulation of immune response and related gene expression profiles in mirror carp (Cyprinus carpio) using selected exotic feed ingredients'
AQUACULTURE
418,
177-184
, DOI
2014
'Effects of dietary β-(1,3)(1,6)-D-glucan supplementation on growth performance, intestinal morphology and haemato-immunological profile of mirror carp (Cyprinus carpio L.)'
J Anim Physiol Anim Nutr (Berl)
98,
(2)
279-289
, DOI
2014
'The Influence of Probiotics on Zebrafish Danio Rerio Innate Immunity and Hepatic Stress'
ZEBRAFISH
11,
(2)
98-106
, DOI
2013
'Dietary synbiotic application modulates Atlantic salmon (Salmo salar) intestinal microbial communities and intestinal immunity'
Fish Shellfish Immunol
35,
(6)
1948-1956
, DOI
2013
'Effect of autoclaved Ulva meal on growth performance, nutrient utilization and fatty acid profile of rainbow trout, Oncorhynchus mykiss'
AQUACULTURE INTERNATIONAL
21,
(3)
605-615
, DOI
2013
'Effects of a dietary β-(1,3)(1,6)-D-glucan supplementation on intestinal microbial communities and intestinal ultrastructure of mirror carp (Cyprinus carpio L.)'
J Appl Microbiol
115,
(5)
1091-1106
, DOI
2013
'Evaluation of feed utilization and growth performance of juvenile striped catfish Pangasianodon hypophthalmus fed diets with varying inclusion levels of corn gluten meal'
AQUACULTURE NUTRITION
19,
(3)
258-266
, DOI
2013
'Genetic diversity of lactic acid bacteria in the intestine of Persian sturgeon fingerlings'
JOURNAL OF APPLIED ICHTHYOLOGY
29,
(3)
494-498
, DOI
2013
'Ingestion of metal-nanoparticle contaminated food disrupts endogenous microbiota in zebrafish (Danio rerio)'
Environ Pollut
174,
157-163
, DOI
2013
'Probiotic Pediococcus acidilactici modulates both localised intestinal- and peripheral-immunity in tilapia (Oreochromis niloticus)'
Fish Shellfish Immunol
35,
(4)
1097-1104
, DOI
2013
'Probiotic, prebiotic and synbiotic applications for the improvement of larval European lobster (Homarus gammarus) culture'
AQUACULTURE
416,
396-406
, DOI
2013
'Replacement of fishmeal with rice protein concentrate in practical diets for European sea bass Dicentrarchus labrax reared at winter temperatures'
AQUACULTURE RESEARCH
44,
(3)
462-471
, DOI
2013
'The effect of different feeding regimes on enzyme activities of gut microbiota in Atlantic cod (Gadus morhua L.)'
AQUACULTURE RESEARCH
44,
(5)
841-846
, DOI
2012
'Biofuel derived yeast protein concentrate (YPC) as a novel feed ingredient in carp diets'
AQUACULTURE
330,
54-62
, DOI
2012
'Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry'
FISH & SHELLFISH IMMUNOLOGY
32,
(2)
316-321
, DOI
2012
'Effect of Dietary Protein Level on Growth Performance and Nitrogen Excretion of the Yellow Tail Cichlid, Pseudotropheus acei'
ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
64,
2012
'Effects of fish oil and partial fish meal substitution with oilseed oils and meals on growth performance, nutrient utilization and health of the rainbow trout Oncorhynchus mykiss'
AQUACULTURE INTERNATIONAL
20,
(3)
481-497
, DOI
2012
'Haemato-immunological and growth response of mirror carp (Cyprinus carpio) fed a tropical earthworm meal in experimental diets'
Fish Shellfish Immunol
32,
(6)
1002-1007
, DOI
2012
'Probiotics Can Induce Follicle Maturational Competence: The Danio rerioCase1'
Biology of Reproduction
86,
(3)
, DOI
2011
'An ex vivo approach to studying the interactions of Pediococcus acidilactici
and Vibrio (Listonella) anguillarum in the anterior intestine of rainbow trout
Oncorhynchus mykiss'
Aquaculture research and development
, DOI
2011
'Assessment of the effects of vegetative and lyophilized Pediococcus acidilactici on growth, feed utilization, intestinal colonization and health parameters of rainbow trout (Oncorhynchus mykiss Walbaum)'
AQUACULTURE NUTRITION
17,
(1)
73-79
, DOI
2011
'Effect of dietary alginic acid on juvenile tilapia (Oreochromis niloticus) intestinal microbial balance, intestinal histology and growth performance'
Cell Tissue Res
344,
(1)
135-146
, DOI
2011
'Effect of dietary Ulva and Spirulina on weight loss and body composition of rainbow trout, Oncorhynchus mykiss (Walbaum), during a starvation period'
J Anim Physiol Anim Nutr (Berl)
95,
(3)
320-327
, DOI
2011
'Effects of Probiotic on Zebrafish Reproduction'
Journal of Aquaculture Research & Development
s1,
, DOI
2011
'Evaluation of Prebiotic and Probiotic Effects on the Intestinal Gut Microbiota and Histology of Atlantic salmon (Salmo salar L.)'
Journal of Aquaculture Research & Development
s1,
, DOI
2011
'Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection'
FISH & SHELLFISH IMMUNOLOGY
31,
(2)
196-201
, DOI
2011
'Identification and characterization of lactic acid bacteria isolated from rainbow trout, Oncorhynchus mykiss (Walbaum), with inhibitory activity against Lactococcus garvieae'
J Fish Dis
34,
(7)
499-507
, DOI
2011
'Microbial manipulations to improve fish health and production--a Mediterranean perspective'
Fish Shellfish Immunol
30,
(1)
1-16
, DOI
2011
'Probiotic and Prebiotic Applications in Aquaculture'
Journal of Aquaculture Research & Development
s1,
, DOI
2011
'The effects of dietary inactive brewer's yeast Saccharomyces cerevisiae var. ellipsoideus on the growth, physiological responses and gut microbiota of juvenile beluga (Huso huso)'
AQUACULTURE
318,
(1-2)
90-94
, DOI
2011
'The effects of oligofructose on growth performance, survival and autochthonous intestinal microbiota of beluga (Huso huso) juveniles'
AQUACULTURE NUTRITION
17,
(5)
498-504
, DOI
2011
'The study of some haematological and serum biochemical parameters of juvenile beluga (Huso huso) fed oligofructose'
FISH PHYSIOLOGY AND BIOCHEMISTRY
37,
(1)
91-96
, DOI
2010
'Assessment of Chlorogloeopsis as a novel microbial dietary supplement for red tilapia (Oreochromis niloticus)'
AQUACULTURE
299,
(1-4)
128-133
, DOI
2010
'Effect of dietary Bacillus spp. and mannan oligosaccharides (MOS) on European lobster (Homarus gammarus L.) larvae growth performance, gut morphology and gut microbiota'
AQUACULTURE
304,
(1-4)
49-57
, DOI
2010
'Effects of mannan oligosaccharide (MOS) supplementation on growth performance, feed utilisation, intestinal histology and gut microbiota of gilthead sea bream (Sparus aurata)'
AQUACULTURE
300,
(1-4)
182-188
, DOI
2010
'Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) I. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria'
AQUACULTURE NUTRITION
16,
(5)
504-510
, DOI
2010
'Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) II. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria postantibiotic treatment'
AQUACULTURE NUTRITION
16,
(5)
496-503
, DOI
2010
'The current status and future focus of probiotic and prebiotic applications for salmonids'
AQUACULTURE
302,
(1-2)
1-18
, DOI
2010
'The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus)'
J Appl Microbiol
109,
(3)
851-862
, DOI
2010
'The effects of inulin on growth factors and survival of the Indian white shrimp larvae and postlarvae (Fenneropenaeus indicus)'
AQUACULTURE RESEARCH
41,
(9)
e348-e352
, DOI
2009
'Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout, Oncorhynchus mykiss (Walbaum)'
J Anim Sci
87,
(10)
3226-3234
, DOI
2009
'Influence of Ulva meal on growth, feed utilization, and body composition of juvenile Nile tilapia (Oreochromis niloticus) at two levels of dietary lipid'
Aquaculture International
17,
(4)
355-361
, DOI
2009
'Microbial community diversity associated with the intestinal mucosa of farmed rainbow trout (Oncoryhnchus mykiss Walbaum)'
AQUACULTURE RESEARCH
40,
(9)
1064-1072
, DOI
2009
'Possible influence of probiotic adhesion to intestinal mucosa on the activity and morphology of rainbow trout ( Oncorhynchus mykiss ) enterocytes'
Aquaculture Research
, DOI
2009
'Preliminary assessment of dietary supplementation of Sangrovit® on red tilapia (Oreochromis niloticus) growth performance and health'
Aquaculture
294,
(1-2)
118-122
, DOI
2009
'Soybean meal alters autochthonous microbial populations, microvilli morphology and compromises intestinal enterocyte integrity of rainbow trout, Oncorhynchus mykiss (Walbaum)'
J Fish Dis
32,
(9)
755-766
, DOI
Books
2014
Aquaculture Nutrition Gut Health, Probiotics and Prebiotics.
Wiley-Blackwell
Chapters
2015
'The fish microbiome and its interactions with mucosal tissues'
Mucosal Health in Aquaculture, 1st Edition
Academic Press
2011
'Dietary Effect of Soybean (Glycine max) Products on Gut Histology and Microbiota of Fish'
Soybean and Nutrition
Rijeka, Croatia
InTech
231-250
Publisher Site
Additional information
Scopus Young Researchers in Biological Sciences: ranked top 5 nationally 2011
Early Career Enterprising Researcher Award, Vice-Chancellor's Enterprise Awards 2010Links
Journal of Aquaculture Research and Development
Fisheries and Aquaculture Journal
Public Library of Science ONE (PLoS ONE)
Aquaculture Research
Aquaculture International
Journal of Aquaculture Feed Science and Nutrition
International Aquafeed