Plymouth Proteomics Research Group

The Plymouth Proteomics Research Group develops and applies advanced proteomic technologies to study human diseases addressing various research questions ranging from biomarker diagnostics to disease mechanisms.

Our key research areas are malaria biology, membrane proteome dynamics, and epigenetic modifications.

We are involved in various internal and external projects with our expertise. If you are interested in proteomics service or a research collaboration, please contact Dr Vikram Sharma from Plymouth University Systems Biology Centre.

Key publications - malaria proteomics:

Lasonder E, Rijpma SR, van Schaijk BC, Hoeijmakers WA, Kensche PR, Gresnigt MS, Italiaander A, Vos MW, Woestenenk R, Bousema T, Mair GR, Khan SM, Janse CJ, Bártfai R, Sauerwein RW (2016). ‘Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression’. Nucleic Acids Res.44, (13) 6087-101. Read this article doi: 10.1093/nar/gkw536.

Bouyer G, Reininger L, Ramdani G, D Phillips L, Sharma V, Egee S, Langsley G, Lasonder E. (2016) ‘Plasmodium falciparum infection induces dynamic changes in the erythrocyte phospho-proteome’  Blood Cells Mol Dis. 58 35-44. Read this article doi: 10.1016/j.bcmd.2016.02.001.

Lasonder E, Green JL, Grainger M, Langsley G, Holder AA (2015). ‘Extensive differential protein phosphorylation as intraerythrocytic Plasmodium falciparum schizonts develop into extracellular invasive merozoites’ . Proteomics 15, (15) 2716-29. Read this article doi: 10.1002/pmic.201400508.

Lasonder E, Green JL, Camarda G, Talabani H, Holder AA, Langsley G, Alano P (2012).’The Plasmodium falciparum schizont phosphoproteome reveals extensive phosphatidylinositol and cAMP-protein kinase A signaling’. J Proteome Res. 11, (11) 5323-37. Read this article doi: 10.1021/pr300557m.

Lasonder E, Ishihama Y, Andersen JS, Vermunt AM, Pain A, Sauerwein RW, Eling WM, Hall N, Waters AP, Stunnenberg HG, Mann M (2002) ‘Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry’ Nature 419, (6906) 537-42. Read this article DOI: 10.1038/nature01111.

Key publications -  membrane proteome dynamics:

C. Ramallo Guevara, O. Philipp, A. Hamann, A. Werner, H.D. Osiewacz, S. Rexroth, M. Rogner, A. Poetsch (2016) 'Global Protein Oxidation Profiling Suggests Efficient Mitochondrial Proteome Homeostasis During Aging' , Mol Cell Proteomics 15, (5) 1692-1709. Read this article DOI: 10.1074/mcp.M115.055616.

C. Trotschel, A. Poetsch (2015) 'Current approaches and challenges in targeted absolute quantification of membrane proteins' Proteomics 15, (5-6) 915-929. Read this article doi: 10.1002/pmic.201400427.

M. Cerletti, R.A. Paggi, C.R. Guevara, A. Poetsch, R.E. De Castro (2015) 'Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii', J Proteomics 121, 1-14. Read this article doi: 10.1016/j.jprot.2015.03.016.

C. Trotschel, S.P. Albaum, D. Wolff, S. Schroder, A. Goesmann, T.W. Nattkemper, A. Poetsch (2012) 'Protein turnover quantification in a multilabeling approach: from data calculation to evaluation' Mol Cell Proteomics 11, (8) 512-526. Read this article doi: 10.1074/mcp.M111.014134.

A. Poetsch, D. Wolters (2008) 'Bacterial membrane proteomics' Proteomics 8, 4100-4122. Read this article doi: 10.1002/pmic.200800273.

Role of epigenetic modifications in cancer:

Research work led by Dr Vikram Sharma focuses on post-translational modifications of histone/non-histone proteins and screening of novel epigenetic inhibitors in various cancer models.

Nucleosome, molecular model

Key publications - cancer epigenetics and proteomics research:

Suárez-Cortés P, Sharma V, Bertuccini L, Costa G, Bannerman NL, Sannella AS, Williamson K, Klemba M, Levashina EA, Lasonder E, Alano P (2016) ‘Comparative proteomics and analysis reveal a role of P. falciparum osmiophilic bodies in malaria parasite transmission’. Mol Cell Proteomics 15, (10) 3243-3255. Read this article DOI: 10.1074/mcp.M116.060681.

Zhou L, Lyons-Rimmer J, Ammoun S, Müller J, Lasonder E, Sharma V, Ercolano E, Hilton D,Taiwo I, Barczyk M, Hanemann CO (2016) ‘The scaffold protein KSR1, a novel therapeutic target for the treatment of Merlin-deficient tumors’ Oncogene 35(26):3443-53. Read this article doi: 10.1038/onc.2015.404.

Sharma V, Tikoo K (2014) ’Stage SpecificQuantitative Changes in the Renal and Urinary Proteome during the Progression and Development of Streptozotocin Induced Diabetic Nephropathy in Rats’ Mol Cell Biochem 388, (1-2):95-111. Read this at DOI: 10.1007/s11010-013-1902-5

Middeljans E, Wan X, Jansen PW, Sharma V, Stunnenberg HG, Logie C (2012) ‘SS18 Together with Animal-Specific Factors Defines Human BAF-Type SWI/SNF Complexes’ PLoS One 7, (3):e33834. Read this at doi:10.1371/journal.pone.0033834.

Shahhoseini M, Favaedi R, Baharvand H, Sharma V, Stunnenberg HG(2010) ‘Evidence for a dynamic role of the linkerhistone variant H1xduring retinoic acid-induced differentiation of NT2 cells’ FEBS Lett. 584, (22): 4661-4664. Read this at DOI: 10.1016/j.febslet.2010.10.041.

Our team