Dr Vikram Sharma
Profiles

Dr Vikram Sharma

Lecturer in Biomedical Sciences

School of Biomedical Sciences (Faculty of Health)

Biography

Biography

Lecturer in Biomedical Sciences & Head of Proteomics Core Laboratory

Qualifications

BSc (Hons) Biochemistry, 2001
MSc (Hons) Biochemistry (Research Masters), 2003
PhD (CSIR Fellow), Pharmacology and Toxicology, 2008 
PgCert in Academic Practice, 2016

Professional membership

BMSS
BBTS Red Cell SIG
Fellow of the HigherEducation Academy, UK

Roles on external bodies

I regularly review research articles from PLOS ONE, Proteomics-Clinical Applications (Wiley), Canadian Journal of Physiology and Pharmacology, Drug Design, Development and Therapy, Current Biomarkers Findings, Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Journal of Blood Medicine (Dovepress). Besides this, I review/assess fellowship applications for The Royal College of Surgeons of England.

I am also an Editorial Board Member in Journal of Natural Science, Biology and Medicine, BEMS Reports and EC Pharmacology & Toxicology Journal.

Key publications

Sequiera Lester G, Sareen N, Sharma V, Choudhary R, Abu Al-Rub E, Moudgil M, Ravandi A & Dhingra S Sequiera Lester G, Sareen N, Sharma V, Choudhary R, Abu Al-Rub E, Moudgil M, Ravandi A & Dhingra S 'Application of “Omics” to Assess the Inter Passage Variability in the Immunoprivilege of Mesenchymal Stem Cells'
Freitas Leal JK, Lasonder E, Sharma V, Schiller J, Fanelli G, Rinalducci S, Brock R & Bosman G (2020) 'Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences' Proteomes 8, (2) 6-6 , DOI Open access
Aldarhami A, Felek A, Sharma V & Upton M (2020) 'Purification and characterization of nisin P produced by a strain of Streptococcus gallolyticus' Journal of Medical Microbiology , DOI Open access
Saeed S, Tremp AZ, Sharma V, Lasonder E & Dessens JT (2020) 'NAD (P) transhydrogenase has vital non‐mitochondrial functions in malaria parasite transmission' EMBO reports 21, (3) , DOI Open access
Sareen N, Sequiera GL, Chaudhary R, Abu-El-Rub E, Chowdhury SR, Sharma V, Surendran A, Moudgil M, Fernyhough P & Ravandi A (2018) 'Early passaging of mesenchymal stem cells does not instigate significant modifications in their immunological behavior' Stem Cells Research and Therapy , DOI Open access
Eymael J, Sharma S, Loeven MA, Wetzels JF, Mooren F, Florquin S, Deegens JK, Willemsen BK, Sharma V & van Kuppevelt TH (2017) 'CD44 is required for the pathogenesis of experimental crescentic glomerulonephritis and collapsing focal segmental glomerulosclerosis' Kidney International 93, (3) 626-642 , DOI Open access
View all publications
Teaching

Teaching

Teaching interests

Seminars on Proteomics and Bioinformatics based Data Analysis. Teaching and training sessions in Mass Spectrometry based Proteomics for undergraduates and postgraduates. 

Lecturing in the following modules:

BIOM3008
BIOM3026
BIOM5006
BIOM5001
BIOM5010
BHCS1004PP
Foundation Year
BHCS2007
BHCS2019
BHCS2002
BHCS3001
Personal tutor: BHCS1001, BHCS1002
BHCS2027

Module lead for BHCS2020 (Human Metabolism) and BIOM5001: Molecular Biology: Genomics, Transcriptomics and Proteomics (Core module in MSc Biomedical Sciences).

Staff serving as external examiners

External reviewer for PhD thesis.

Research

Research

Research interests

I am running the Proteomics Core Laboratory located within the Derriford Research Facility. We collaborate on various projects employing LC-MS based Proteomics and Bioinformatic workflows to address challenging research questions. Besides this, I also provide specialist advice on experimental designs for a wide range of research projects.

My research primarily focusses on development of customised LC-MS based Proteomic Workflows for a wide range of applications. I have keen interest in epigenetic post translational modifications and their role in disease pathology, cancer and toxic responses.

 

PhD students:

1. Mr Raed Garout: New methods for detection of Down's syndrome pregnancies. (Co-Supervisor)

2. Ms Yeasmin Akhter: Proteomics based profiling of meningiomas. (Co-Supervisor)

 

Past PhD students:

Dr Nizar Saeedi: Investigating Virulence of ST127 Uropathogenic Escherichia coli.

Ms Charlotte Crowther: Analytical, toxicogenomics and modelling approaches to determine the impacts of radionuclides and metals. 

 

Current masters students:

Mr Jake Pennels Dovey and Mr Matthew Borbon: Deciphering the mode of action of new generation Epigenetic modifiers in Cancer. 

 

There are 8 current undergraduate students on projects investigating novel drug targets in Non-Small Cell Lung Carcinoma (NSCLC) and B-ALL.

 

Previous UK and EU Research exchange trainees:

Research Assistant: Ms Avinisha Lal (Role of New generation of Epi-Drugs in Cancer)

Erasmus Exchange Student: Ms Aurore Hebras (University of Poitiers, France)

 

Previous undergraduate, placement and masters students:

Mr Jake Serle: Role of Epigenetic inhibitors in non small cell lung carcinoma. 

Ms Lucinda Burt: Deciphering the mode of action of new generation Epigenetic modifiers in Non Small Cell Lung Carcinoma 

Mr Daniel Machin; Novel Epidrugs in NSCLC

Ms Maisie Fox: Epigenetic mechanisms underlying Acute Lymphoid Leukemia. 

Mr Jordan Martin: Role of Hybrid Histone Deacetylase Inhibitors in Acute Lymphoid Leukemia

Mr Adam North: Proteomic profiling to reveal mode of action of UVI5008 in B-ALL

Ms Abena Yeboah: Proteomic based screen to investigate mode of action of NCE's in B-ALL

Ms Jasmin Mirzayan: Role of Hybrid Epi-Drugs in B-ALL.

Mr Ojieriakhi Ede-Ehizode

Mr Toby Huxtable

 

Potential new positions:

Self funded PhD/ResM applicants are encouraged to apply.

Research groups

Grants & contracts

Projects running in the facility:
RCUK-CONICYT ( Co-Investigator)
EU-FP7 Nanosolutions 
EU-FP7-SysVasc
EU-H2020 Transat ( Co-Investigator) 
Royal Society International Exchange Award (Principal Investigator: 2018-2020)

Publications

Publications

Key publications

Sequiera Lester G, Sareen N, Sharma V, Choudhary R, Abu Al-Rub E, Moudgil M, Ravandi A & Dhingra S Sequiera Lester G, Sareen N, Sharma V, Choudhary R, Abu Al-Rub E, Moudgil M, Ravandi A & Dhingra S 'Application of “Omics” to Assess the Inter Passage Variability in the Immunoprivilege of Mesenchymal Stem Cells'
Freitas Leal JK, Lasonder E, Sharma V, Schiller J, Fanelli G, Rinalducci S, Brock R & Bosman G (2020) 'Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences' Proteomes 8, (2) 6-6 , DOI Open access
Aldarhami A, Felek A, Sharma V & Upton M (2020) 'Purification and characterization of nisin P produced by a strain of Streptococcus gallolyticus' Journal of Medical Microbiology , DOI Open access
Saeed S, Tremp AZ, Sharma V, Lasonder E & Dessens JT (2020) 'NAD (P) transhydrogenase has vital non‐mitochondrial functions in malaria parasite transmission' EMBO reports 21, (3) , DOI Open access
Sareen N, Sequiera GL, Chaudhary R, Abu-El-Rub E, Chowdhury SR, Sharma V, Surendran A, Moudgil M, Fernyhough P & Ravandi A (2018) 'Early passaging of mesenchymal stem cells does not instigate significant modifications in their immunological behavior' Stem Cells Research and Therapy , DOI Open access
Eymael J, Sharma S, Loeven MA, Wetzels JF, Mooren F, Florquin S, Deegens JK, Willemsen BK, Sharma V & van Kuppevelt TH (2017) 'CD44 is required for the pathogenesis of experimental crescentic glomerulonephritis and collapsing focal segmental glomerulosclerosis' Kidney International 93, (3) 626-642 , DOI Open access

Key publications are highlighted

Journals
Articles
Tremp AZ, Saeed S, Sharma V, Lasonder E & Dessens JT (2020) 'Plasmodium berghei LAPs form an extended protein complex that facilitates crystalloid targeting and biogenesis' Journal of Proteomics 227, 103925-103925 , DOI Open access
Green JL, Wu Y, Encheva V, Lasonder E, Prommaban A, Kunzelmann S, Christodoulou E, Grainger M, Truongvan N & Bothe S (2020) 'Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development' PLOS Pathogens 16, (6) e1008640-e1008640 , DOI Open access
Freitas Leal JK, Lasonder E, Sharma V, Schiller J, Fanelli G, Rinalducci S, Brock R & Bosman G (2020) 'Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences' Proteomes 8, (2) 6-6 , DOI Open access
Aldarhami A, Felek A, Sharma V & Upton M (2020) 'Purification and characterization of nisin P produced by a strain of Streptococcus gallolyticus' Journal of Medical Microbiology , DOI Open access
Saeed S, Tremp AZ, Sharma V, Lasonder E & Dessens JT (2020) 'NAD (P) transhydrogenase has vital non‐mitochondrial functions in malaria parasite transmission' EMBO reports 21, (3) , DOI Open access
Barranger A, Langan LM, Sharma V, Rance GA, Aminot Y, Weston NJ, Akcha F, Moore MN, Arlt VM & Khlobystov AN (2019) 'Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels' Nanomaterials 9, (7) 0-0 , DOI Open access
Sequiera GL, Sareen N, Sharma V, Surendran A, Abu-El-Rub E, Ravandi A & Dhingra S (2019) 'High throughput screening reveals no significant changes in protein synthesis, processing, and degradation machinery during passaging of mesenchymal stem cells' Canadian Journal of Physiology and Pharmacology 97, (6) 536-543 , DOI
Dunn J, Ferluga S, Sharma V, Futschik M, Hilton DA, Adams CL, Lasonder E & Hanemann CO (2018) 'Proteomic analysis discovers the differential expression of novel proteins and phosphoproteins in meningioma including NEK9, HK2 and SET and deregulation of RNA metabolism' EBioMedicine Author Site , DOI Open access
Sareen N, Sequiera GL, Chaudhary R, Abu-El-Rub E, Chowdhury SR, Sharma V, Surendran A, Moudgil M, Fernyhough P & Ravandi A (2018) 'Early passaging of mesenchymal stem cells does not instigate significant modifications in their immunological behavior' Stem Cells Research and Therapy , DOI Open access
Eymael J, Sharma S, Loeven MA, Wetzels JF, Mooren F, Florquin S, Deegens JK, Willemsen BK, Sharma V & van Kuppevelt TH (2017) 'CD44 is required for the pathogenesis of experimental crescentic glomerulonephritis and collapsing focal segmental glomerulosclerosis' Kidney International 93, (3) 626-642 , DOI Open access
Cunliffe M, Hollingsworth A, Bain C, Sharma V & Taylor JD (2017) 'Algal polysaccharide utilisation by saprotrophic planktonic marine fungi' Fungal Ecology 30, 135-138 , DOI Open access
Tremp AZ, Sharma V, Carter V, Lasonder E & Dessens JT (2017) 'LCCL protein complex formation in Plasmodium is critically dependent on LAP1' Mol Biochem Parasitol Author Site , DOI Open access
Bassiri K, Ferluga S, Sharma V, Syed N, Adams CL, Lasonder E & Hanemann CO (2017) 'Global Proteome and Phospho-proteome Analysis of Merlin-deficient Meningioma and Schwannoma Identifies PDLIM2 as a Novel Therapeutic Target' EBioMedicine , DOI Open access
Suárez-Cortes P, Sharma V, Bertuccini L, Costa G, Bannerman NL, Sannella AR, Williamson K, Klemba M, Levashina EA & Lasonder E (2016) 'Comparative proteomics and functional analysis reveal a role of plasmodium falciparum osmiophilic bodies in malaria parasite transmission' Molecular and Cellular Proteomics , DOI Open access
Bouyer G, Reininger L, Ramdani G, D Phillips L, Sharma V, Egee S, Langsley G & Lasonder E (2016) 'Plasmodium falciparum infection induces dynamic changes in the erythrocyte phospho-proteome' Blood cells, molecules & diseases 58, 35-44 Open access
Zhou L, Lyons-Rimmer J, Ammoun S, Müller J, Lasonder E, Sharma V, Ercolano E, Hilton D, Taiwo I & Barczyk M (2015) 'The scaffold protein KSR1, a novel therapeutic target for the treatment of Merlin-deficient tumors' Oncogene 35, (26) 3443-3453 , DOI Open access
Sharma V & Tikoo K (2014) 'Stage-specific quantitative changes in renal and urinary proteome during the progression and development of streptozotocin-induced diabetic nephropathy in rats' Mol Cell Biochem 388, (1-2) 95-111 Publisher Site
Costessi A, Mahrour N, Sharma V, Stunnenberg R, Stoel MA, Tijchon E, Conaway JW, Conaway RC & Stunnenberg HG (2012) 'The human EKC/KEOPS complex is recruited to Cullin2 ubiquitin ligases by the human tumour antigen PRAME' Plos One
Middeljans E, Wan X, Jansen PW, Sharma V, Stunnenberg HG & Logie C (2012) 'SS18 together with animal-specific factors defines human BAF-type SWI/SNF complexes' Plos One
Shahhoseini M, Favaedi R, Baharvand H, Sharma V & Stunnenberg HG (2010) 'Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells' FEBS Letters 4661-4664
Tikoo K, Singh K, Kabra D, Sharma V & Gaikwad AB (2008) 'Change in histone H3 phosphorylation, MAP kinase p38, SIR 2 and p53 expression by resveratrol in preventing streptozotocin induced type I diabetic nephropathy' Free Radical Research 397-404
Tikoo K, Bhatt DK, Gaikwad AB, Sharma V & Kabra DG (2007) 'Differential effects of tannic acid on cisplatin induced nephrotoxicity in rats' FEBS Letters 2027-2035
Tikoo K, Tripathi DN, Kabra DG, Sharma V & Gaikwad AB (2007) 'Intermittent fasting prevents the progression of type I diabetic nephropathy in rats and changes the expression of Sir2 and p53' FEBS Letters 1071-1078
Katoch CDS, Deoskar RB, Barthwal MS, Sharma SK, Sharma V & Bhattacharya P (2006) 'Bronchiolitis Obliterans with Organising Pneumonia presenting as Non Resolving Pneumonia' Medical Journal Armed Forces India 62, (4) 378-380 , DOI
Conference Papers
Sharma V, de Lera A & Stunnenberg HG (2013) 'Quantitative mass spectrometric analysis reveals the target acetylome of epigenetic inhibitor UVI5008' British Society for Proteome Research 2-/-1/20132-/-1/2013
Sharma V, de Lera A & Stunnenberg HG (2013) 'Quantitative mass spectrometric analysis reveals the target acetylome of epigenetic inhibitor UVI5008' British Society for Proteome Research 2-/-1/20132-/-1/2013
Posters
Sequiera Lester G, Sareen N, Sharma V, Choudhary R, Abu Al-Rub E, Moudgil M, Ravandi A & Dhingra S Sequiera Lester G, Sareen N, Sharma V, Choudhary R, Abu Al-Rub E, Moudgil M, Ravandi A & Dhingra S 'Application of “Omics” to Assess the Inter Passage Variability in the Immunoprivilege of Mesenchymal Stem Cells'
Sharma V & Tikoo K Sharma V & Tikoo K 'Proteomic Approach to Identify Stage Specific Biomarkers in Streptozotocin Induced Diabetic Nephropathy'
Personal

Personal

Conferences organised

IBMS accredited CPD event: Workshop on "Hands On Proteomics and Data Analysis" (2014)

Other academic activities

Supervision of undergraduate, postgraduate and PhD students. I also offer summer placement, masters and undergraduate research projects. External reviewer for PhD dissertations.

Links

http://scholar.google.co.uk/citations?user=lfBPnqQAAAAJ&hl=en&oi=ao