BSc (Hons) Conservation Biology fieldtrip to Costa Rica: view of rope bridge in the jungle
Groundbreaking new research suggests the Earth harbours a minimum of between 14 and 30 million insect species – far more than the long-standing global estimate of approximately six million.
The study was carried out by an international team of experts including Dr Robert Puschendorf, who is originally from Costa Rica but has spent the last 13 years working at the University of Plymouth.
It is based on an analysis of more than 1.6 million DNA-barcoded insects collected from Área de Conservación Guanacaste (ACG), a renowned UNESCO World Heritage Site in Costa Rica, and represents one of the most comprehensive efforts ever undertaken to document tropical insect diversity.
It combined multiple collection techniques, ecological observations, DNA barcoding, and powerful statistics with ACG estimates and they were then cross-referenced to multiple different taxa (including trees, amphibians and moths).
As a result, the researchers found that different methods consistently revealed an enormous number of cryptic species – underscoring how much biodiversity remains hidden: their conservative estimate suggests that between 93-97% of insect species remain nameless.
Beyond the striking global estimate itself, the study carries an important broader message: humanity still knows remarkably little about the vast majority of species with which we share the planet.
Dr Puschendorf, Associate Professor in Conservation Biology at the University, contributed analysis estimating the number of amphibian species in ACG, a place he has known since his childhood, and the creatures that started his personal journey into a career in conservation.
For the new study, he highlighted how populations have changed over recent decades as a result of issues such as climate change and deforestation and the need for greater research before further species are lost forever.

There is always a big debate about how much life exists on our planet – and the argument we, as conservationists, make is that if you do not know what you have, how can you manage it.

Diversity is really hard to quantify, but what this new study shows is that we have completely underestimated the insects. While it is focused on Costa Rica, a place very close to my heart, these same techniques can be applied anywhere in the world – including here in the UK. Only by doing that will we truly understand the species we share our planet with, the species we have already lost and how we can best protect the survivors going forward.

Robert PuschendorfDr Robert Puschendorf
Associate Professor in Conservation Biology

Read more about Dr Robert Puschendorf's work to study the amazing biodiversity of Costa Rica: Under the microscope: Studying the effects of climate change in Costa Rica 
Alex Edwards' picture of a powdered glass frog in the Costa Rican rainforest (Credit: Alex Edwards, University of Plymouth)
The new research was led by scientists at the University of Cornell, University of Colorado and the University of Kentucky. But at its heart lies more than 30 years of continuous biodiversity inventory work by internationally renowned scientists and Costa Rican biodiversity specialists who have spent decades rearing caterpillars, maintaining field sampling systems (including Malaise traps), documenting ecological relationships, and helping reveal the immense complexity of tropical ecosystems.
The paper particularly highlights the extraordinary diversity of tiny parasitic wasps, many of which are highly specialised to specific caterpillar hosts and habitats. These relationships could only be uncovered by complementing the long-term caterpillar rearing program established in ACG with DNA barcoding and taxonomic expertise.
The research also reflects ACG’s longstanding commitment to democratising biodiversity knowledge through DNA barcoding, an effort supported over two decades through collaborations among Costa Rican and international scientists, institutions, and supporters of the bioinventory.
As well as his work in Plymouth, Dr Puschendorf is an adviser Guanacaste Dry Forest Conservation Fund (GDFCF), which supports the ongoing biodiversity inventory, conservation, restoration, and scientific research efforts of Área de Conservación Guanacaste in northwestern Costa Rica.

This work stands as an incredible tribute to the parataxonomists of ACG. Their dedication, field knowledge, and passion created the empirical foundation that made this research possible. For decades, ACG and GDFCF have supported the integration of this expertise with scientists around the world in order to create a globally unprecedented understanding of biological diversity. Species are the actors in the Earth’s environmental dramas. This study suggests that, while we are embedded within this ecological theatre; we don’t yet know most of the players in the play.

Dr Alex Smith
Associate Professor at the University of Guelph, Canada, and GDFCF Board Member
  • The full study – Colwell et al: Constructing a lower-bound estimate of the global number of insect species on a hyperdiverse empirical foundation – is published in Proceedings of the National Academy of Sciences (PNAS), DOI: 10.1073/pnas.2524283123.
 

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Students listening to their tutor in the jungle on BSc (Hons) Conservation Biology fieldtrip to Costa Rica