Dr Tracey Madgett
Profiles

Dr Tracey Madgett

Senior Research Fellow - Postgenomics Technologies

School of Biomedical & Healthcare Sciences (Plymouth University Peninsula Schools of Medicine and Dentistry)

Role

Laboratory Manager for Prof. Neil Avent's Research Group (Blood Diagnostics), the Centre for Biomedical Research Laboratory (Davy 802) and the Plymouth University Systems Biology Centre (Davy 2 North)






Qualifications

B.A. Hons. Natural Sciences - Cambridge University (Newnham College), 1996

M.A. -  Cambridge University, 2000 

Ph.D. - The regulation of cyclooxygenase-2 in an in vitro model of colorectal tumorigenesis and mechanisms of action of potential chemopreventive agents.  Bristol University, 2000 

Post-Graduate Certificate in Academic Practice (PGCAP) - Plymouth University, 2015

Background

Prior to joining Plymouth University in 2010, Tracey completed post-doctoral studies at Bath University (2000-2003) and the University of the West of England, Bristol (2003-2010).  She became proficient in a variety of laboratory techniques and alongside her own research was able to support undergraduate students during their final year projects.  Cancer research made way for blood group genotyping research and then non-invasive prenatal diagnosis research (with the group of Prof Neil Avent).

Since arriving in Plymouth, Tracey has managed the day-to-day running of the laboratory of Prof Neil Avent (including supervision of Ph.D. students and undergraduates).  In 2011, she was heavily involved in the design of the refurbishment of the laboratories for the Plymouth University Systems Biology Centre (genomics and proteomics facilities) and is now the Director of the Centre. 

 

Professional membership

Fellow, Higher Education Academy

Teaching interests

Main teaching areas: non-invasive prenatal diagnosis, blood groups and genomics/proteomics technologies.
Personal Tutor for Biomedical Sciences
Foundation year: BIO013
Second year: BHCS2002 (Evidence-based practice in Biomedical Science), BHCS2007 (Diagnostic and Clinical Biomedicine) and BHCS2019 (Methods in Human Biosciences)
Third year: BHCS3001 (Personal Research Project; Module Co-Lead), BHCS3004 (Specialist Biochemistry and Screening), BHCS3008 (Current developments in Biomedical Science), BHCS3010/BHCS3014 (Haematology and Transfusion Medicine/Reproductive Science) 
Masters level: BIOM5010 (Medical Genomics and Personalised Medicine), BIOM5001 (Molecular Biology: Genomics, Transcriptomics and Proteomics), BIOM5006 (Research Project)

Research interests

Molecular diagnostics in the fields of transfusion medicine and non-invasive prenatal diagnosis, primarily blood group genotyping (with a particular interest in RHD genetics), free fetal DNA enrichment methods and finding new protein biomarkers for Down Syndrome in maternal plasma.  Research focus involves using technologies such as next generation sequencing, digital PCR and mass spectrometry/proteomics (including difference in gel electrophoresis, DIGE).

UoP Research Group Membership

Biomedical Research group

Key publications are highlighted

Journals
Articles
Sillence KA, Halawani AJ, Tounsi WA, Clarke KA, Kiernan M, Madgett TE & Avent ND 2017 'Rapid RHD Zygosity Determination Using Digital PCR' Clinical Chemistry Author Site , DOI PEARL
Sillence KA, Roberts LA, Hollands HJ, Thompson HP, Kiernan M, Madgett TE, Welch CR & Avent ND 2015 'Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR' Clin Chem 61, (11) 1399-1407 Author Site , DOI PEARL
Li B, Pan G, Avent ND, Lowry RB, Madgett TE & Waines PL 2015 'Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection' Biosensors and Bioelectronics 72, 313-319 , DOI PEARL
Sillence KA, Madgett TE, Roberts LA, Overton TG & Avent ND 2013 'Non-invasive screening tools for Down's Syndome: A review' Diagnostics 3, (2) 291-314 , DOI
Haer-Wigman L, Veldhuisen B, Jonkers R, Lodén M, Madgett TE, Avent ND, de Haas M & van der Schoot CE 2013 'RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification' Transfusion 53, (7) 1559-1574 Author Site , DOI
Heywood W, Mills K, Wang D, Hogg J, Madgett TE, Avent ND & Chitty LS 2012 'Identification of new biomarkers for Down's syndrome in maternal plasma' J Proteomics 75, (9) 2621-2628 Author Site , DOI
Webb A, Madgett T, Miran T, Sillence K, Kaushik N, Kiernan M & Avent N 2012 'Non invasive prenatal diagnosis of aneuploidy: next generation sequencing or fetal DNA enrichment?' Balkan J Med Genet 15, (Suppl) 17-26 Author Site , DOI
Heywood W, Wang D, Madgett TE, Avent ND, Eaton S, Chitty LS & Mills K 2012 'The development of a peptide SRM-based tandem mass spectrometry assay for prenatal screening of Down syndrome' J Proteomics 75, (11) 3248-3257 Author Site , DOI
Heywood WE, Madgett TE, Wang D, Wallington A, Hogg J, Mills K & Avent ND 2011 '2D DIGE analysis of maternal plasma for potential biomarkers of Down Syndrome' Proteome Sci 9, Author Site , DOI
Wigman L, Veldhuisen B, Soussan AA, Avent ND, Madgett TE, Maddocks DG, de Haas M & van der Schoot CE 2010 'DEVELOPMENT OF A MULTIPLEX LIGAND-DEPENDENT PROBE AMPLIFICATION (MLPA) ASSAY TO DETERMINE VARIANTS IN THE RHD AND RHCE GENE' VOX SANGUINIS 99, 506-506 Author Site
Jeremy KP, Plummer ZE, Head DJ, Madgett TE, Sanders KL, Wallington A, Storry JR, Gilsanz F, Delaunay J & Avent ND 2009 '4.1R-deficient human red blood cells have altered phosphatidylserine exposure pathways and are deficient in CD44 and CD47 glycoproteins' Haematologica 94, (10) 1354-1361 Author Site , DOI PEARL
Avent ND, Madgett TE, Maddocks DG & Soothill PW 2009 'Cell-free fetal DNA in the maternal serum and plasma: current and evolving applications' Curr Opin Obstet Gynecol 21, (2) 175-179 Author Site , DOI
Avent ND, Martinez A, Flegel WA, Olsson ML, Scott ML, Nogués N, Písacka M, Daniels GL, Muñiz-Diaz E & Madgett TE 2009 'The Bloodgen Project of the European Union, 2003-2009' Transfus Med Hemother 36, (3) 162-167 Author Site , DOI
Maddocks DG, Alberry MS, Attilakos G, Madgett TE, Choi K, Soothill PW & Avent ND 2009 'The SAFE project: towards non-invasive prenatal diagnosis' Biochem Soc Trans 37, (Pt 2) 460-465 Author Site , DOI
Avent ND, Plummer ZE, Madgett TE, Maddocks DG & Soothill PW 2008 'Post-genomics studies and their application to non-invasive prenatal diagnosis' Semin Fetal Neonatal Med 13, (2) 91-98 Author Site , DOI
Avent ND, Martinez A, Flegel WA, Olsson ML, Scott ML, Nogués N, Písacka M, Daniels G, van der Schoot E & Muñiz-Diaz E 2007 'The BloodGen project: toward mass-scale comprehensive genotyping of blood donors in the European Union and beyond' Transfusion 47, (1 Suppl) 40S-46S Author Site , DOI
Avent ND, Madgett TE, Lee ZE, Head DJ, Maddocks DG & Skinner LH 2006 'Molecular biology of Rh proteins and relevance to molecular medicine' Expert Rev Mol Med 8, (13) 1-20 Author Site , DOI
Fox BC, Crew TE & Welham MJ 2005 'Phosphoinositide 3-kinases can act independently of p27Kip1 to regulate optimal IL-3-dependent cell cycle progression and proliferation' Cell Signal 17, (4) 473-487 Author Site , DOI
Charalambous M, Smith FM, Bennett WR, Crew TE, Mackenzie F & Ward A 2003 'Disruption of the imprinted Grb10 gene leads to disproportionate overgrowth by an Igf2-independent mechanism' Proc Natl Acad Sci U S A 100, (14) 8292-8297 Author Site , DOI
Bennett WR, Crew TE, Slack JMW & Ward A 2003 'Structural-proliferative units and organ growth: effects of insulin-like growth factor 2 on the growth of colon and skin' Development 130, (6) 1079-1088 Author Site
Crew TE, Elder DJ & Paraskeva C 2000 'A cyclooxygenase-2 (COX-2) selective non-steroidal anti-inflammatory drug enhances the growth inhibitory effect of butyrate in colorectal carcinoma cells expressing COX-2 protein: regulation of COX-2 by butyrate' Carcinogenesis 21, (1) 69-77 Author Site
Elder DJ, Halton DE, Crew TE & Paraskeva C 2000 'Apoptosis induction and cyclooxygenase-2 regulation in human colorectal adenoma and carcinoma cell lines by the cyclooxygenase-2-selective non-steroidal anti-inflammatory drug NS-398' Int J Cancer 86, (4) 553-560 Author Site
Conference Papers
Madgett TE, Maddocks DG, Nygren A, Choi K & Avent ND 2005 'Rapid molecular genotyping for RHD using multiplex ligation-dependent probe amplification technology' 129A-129A
Smith F, Charalambous M, Bennett W, Crew T, Mackenzie F & Ward A 2004 'Disruption of the imprinted Grb10 gene in mice leads to disproportionate overgrowth with effects on metabolism' 117-118
Charalambous M, Bennett WR, Smith F, Crew TE, Mackenzie F & Ward A 2003 'Growth regulation by Grb10' 236-236
Paraskeva C, Hague A, Crew T & Elder DJE 1999 'Chemoprevention of colon cancer: diet NSAIDs and apoptosis' 568-568
Internet Publications
Madgett TE 2008 'Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader'