- FF122, Derriford Research Facility, 14 Research Way, Plymouth Science Park, Plymouth, PL6 8BU
- +44 1752 584894
- tracey.madgett@plymouth.ac.uk
Profiles
Dr Tracey Madgett
Lecturer in Molecular Biology
School of Biomedical Sciences (Faculty of Health)
Biography
Biography
Programme Lead for BSc (Hons) Biomedical Science
Lead for the Blood Diagnostics Research Group
Qualifications
B.A. Hons. Natural Sciences - Cambridge University (Newnham College), 1996
M.A. - Cambridge University, 2000
Ph.D. - The regulation of cyclooxygenase-2 in an in vitro model of colorectal tumorigenesis and mechanisms of action of potential chemopreventive agents. Bristol University, 2000
Post-Graduate Certificate in Academic Practice (PGCAP) - Plymouth University, 2015
Background
Prior to joining Plymouth University in 2010, Tracey completed post-doctoral studies at Bath University (2000-2003) and the University of the West of England, Bristol (2003-2010). She became proficient in a variety of laboratory techniques and alongside her own research was able to support undergraduate students during their final year projects. Cancer research made way for blood group genotyping research and then non-invasive prenatal diagnosis research (with the group of Prof Neil Avent).
Since arriving in Plymouth, Tracey has managed the day-to-day running of the laboratory of Prof Neil Avent (including supervision of Ph.D. students and undergraduates). In 2011, she was heavily involved in the design of the refurbishment of the laboratories for the Plymouth University Systems Biology Centre (genomics and proteomics facilities) and is now the Director of the Centre. Since Neil Avent's retirement in March 2018, Tracey is now the group lead for the Blood Diagnostics Research Group, based at the Derriford Research Facility. Tracey became Programme Lead for BSc (Hons) Biomedical Science in May 2021.
Professional membership
Fellow, Higher Education Academy
Key publications
(2015) 'Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR' Clinical Chemistry 61, (11) 1399-1407 , DOI Open access
Teaching
Teaching
Teaching interests
Main teaching areas: molecular biology, non-invasive prenatal diagnosis, blood groups and genomics/proteomics technologies.
Personal Tutor for Biomedical Sciences (BHCS1011, BHCS2027 and BHCS3031)
Foundation year: FMD001 (Molecules to Cells), FMD002 (Introduction to Human Physiology; Module lead) and BHCS002 (Current Developments in Human Biology and Biomedical Sciences)
First year: BHCS1005 (Human Disease)
Second year: BHCS2002 (Evidence-based practice in Biomedical Science), BHCS2003 (Genetic Continuity and Diversity), BHCS2005 (Clinical Haematology and Biochemistry), BHCS2019 (Methods in Human Biosciences) and BHCS2032 (Contemporary Healthcare Science Practice)
Third year: BHCS3001 (Personal Research Project; Module Co-Lead), BHCS3004 (Specialist Biochemistry and Screening), BHCS3005 (Transplantation and Transfusion Science), BHCS3008 (Current Developments in Biomedical Science; Module Lead) and BHCS3014 (Reproductive Science)
Masters level: BIOM5001 (Molecular Biology: Genomics, Transcriptomics and Proteomics), BIOM5005 (Project Design and Development) and BIOM5006 (Research Project)
Research
Research
Research interests
Molecular diagnostics in the fields of transfusion medicine and non-invasive prenatal diagnosis, primarily blood group genotyping (with a particular interest in RHD genetics), free fetal DNA enrichment methods and finding new protein biomarkers for Down Syndrome in maternal plasma. Research focus involves using technologies such as next generation sequencing, digital PCR and mass spectrometry/proteomics.
Current interests include investigating the role of CD47 in colorectal cancer cell lines.
UoP Research Group MembershipBiomedical Research group
Research groups
Grants & contracts
Northcott Devon Medical Foundation (2019-2021) (£10K)
Publications
Publications
Key publications
(2015) 'Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR' Clinical Chemistry 61, (11) 1399-1407 , DOI Open access
Key publications are highlighted
Journals
Articles
(2023) 'RHD molecular analysis—from discovery to next generation sequencing' Annals of Blood Publisher Site , DOI Open access
(2022) 'First Trimester Noninvasive Prenatal Diagnosis of Maternally Inherited Beta-Thalassemia Mutations' Clinical Chemistry , DOI Open access
(2022) 'Rh blood group D antigen genotyping using a portable nanopore based sequencing device: proof of principle' Clinical Chemistry , DOI Open access
(2020) 'Next-generation sequencing of 35 RHD variants in 16 253 serologically D− pregnant women in the Finnish population' Blood Advances 4, (20) 4994-5001 , DOI Open access
(2019) 'Tounsi WA, Madgett TE, Avent ND. Complete RHD next-generation sequencing: establishment of reference RHD alleles. Blood Adv. 2018;2(20):2713-2723' Blood Advances 3, (2) 120-120 , DOI
(2018) 'Complete RHD next-generation sequencing: establishment of reference RHD alleles' Blood Advances 2, (20) 2713-2723 , DOI Open access
(2015) 'Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR' Clinical Chemistry 61, (11) 1399-1407 , DOI Open access
(2015) 'Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection' Biosensors and Bioelectronics 72, 313-319 , DOI Open access
(2013) 'RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification' Transfusion 53, (7) 1559-1574 , DOI
(2012) 'Non Invasive Prenatal Diagnosis of Aneuploidy: Next Generation Sequencing or Fetal DNA Enrichment?' Balkan Journal of Medical Genetics 15, (Supplement) 17-26 , DOI Open access
(2012) 'The development of a peptide SRM-based tandem mass spectrometry assay for prenatal screening of Down syndrome' J Proteomics 75, (11) 3248-3257 , DOI
(2012) 'Identification of new biomarkers for Down's syndrome in maternal plasma' J Proteomics 75, (9) 2621-2628 , DOI
(2011) '2D DIGE analysis of maternal plasma for potential biomarkers of Down Syndrome' Proteome Sci 9, , DOI Open access
(2010) 'DEVELOPMENT OF A MULTIPLEX LIGAND-DEPENDENT PROBE AMPLIFICATION (MLPA) ASSAY TO DETERMINE VARIANTS IN THE RHD AND RHCE GENE' VOX SANGUINIS 99, 506-506
(2009) '4.1R-deficient human red blood cells have altered phosphatidylserine exposure pathways and are deficient in CD44 and CD47 glycoproteins' Haematologica 94, (10) 1354-1361 , DOI Open access
(2009) 'Cell-free fetal DNA in the maternal serum and plasma: current and evolving applications' Curr Opin Obstet Gynecol 21, (2) 175-179 , DOI
(2009) 'The SAFE project: towards non-invasive prenatal diagnosis' Biochem Soc Trans 37, (Pt 2) 460-465 , DOI
(2009) 'The Bloodgen Project of the European Union, 2003-2009' Transfus Med Hemother 36, (3) 162-167 , DOI
(2008) 'Post-genomics studies and their application to non-invasive prenatal diagnosis' Semin Fetal Neonatal Med 13, (2) 91-98 , DOI
(2007) 'The BloodGen project: toward mass-scale comprehensive genotyping of blood donors in the European Union and beyond' Transfusion 47, (1 Suppl) 40S-46S , DOI
(2006) 'Molecular biology of Rh proteins and relevance to molecular medicine' Expert Rev Mol Med 8, (13) 1-20 , DOI
(2005) 'Phosphoinositide 3-kinases can act independently of p27Kip1 to regulate optimal IL-3-dependent cell cycle progression and proliferation' Cell Signal 17, (4) 473-487 , DOI
(2003) 'Disruption of the imprinted Grb10 gene leads to disproportionate overgrowth by an Igf2-independent mechanism' Proc Natl Acad Sci U S A 100, (14) 8292-8297 , DOI
(2003) 'Structural-proliferative units and organ growth: effects of insulin-like growth factor 2 on the growth of colon and skin' Development 130, (6) 1079-1088 , DOI
(2000) 'Apoptosis induction and cyclooxygenase-2 regulation in human colorectal adenoma and carcinoma cell lines by the cyclooxygenase-2-selective non-steroidal anti-inflammatory drug NS-398' Int J Cancer 86, (4) 553-560 , DOI
(2000) 'A cyclooxygenase-2 (COX-2) selective non-steroidal anti-inflammatory drug enhances the growth inhibitory effect of butyrate in colorectal carcinoma cells expressing COX-2 protein: regulation of COX-2 by butyrate' Carcinogenesis 21, (1) 69-77 , DOI
Conference Papers
(2015) 'Next‐generation sequencing: academic overkill or high‐resolution routine blood group genotyping?' Wiley 250-256 , DOI
(2005) 'Rapid molecular genotyping for RHD using multiplex ligation-dependent probe amplification technology' 129A-129A
(2004) 'Disruption of the imprinted <i>Grb10</i> gene in mice leads to disproportionate overgrowth with effects on metabolism' 117-118
(2003) 'Growth regulation by <i>Grb10</i>' 236-236
(1999) 'Chemoprevention of colon cancer: diet NSAIDs and apoptosis' 568-568
Other Publications
(2008) Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader. Publisher Site