Dr Tina Joshi
Profiles

Dr Tina Joshi

Lecturer in Molecular Microbiology

Plymouth University Peninsula Schools of Medicine and Dentistry

Role

Lecturer in Molecular Microbiology (2017-)

Teaching and Research

ORCID ID: 0000-0002-5965-4055

After completing BSc and PhD studies at Cardiff University, I undertook several postdoctoral roles from 2012-2017 before commencement of a Lectureship at the University of Plymouth.

Qualifications

2012 PhD Pharmacy "Pathogenicity and a bedside real time detection assay for C.  difficile in the faeces of hospitalised patients". Cardiff University

2008 BSc Microbiology Hons. Cardiff University

Professional membership

Associate Fellow Higher Education Academy

Member of Royal Society of Biology (MRSB)

Member of Society for Applied Microbiology

Member of Microbiology Society

STEM Ambassador

Roles on external bodies

Reviewer:
Journal of Applied Microbiology
Letters in Applied Microbiology
BMC Microbiology
American Journal of Infection Control

Teaching interests

Molecular microbiology in infection disease, clinical microbiology, molecular diagnosis of microbial infections and bacterial genomics. This includes supervision of PG and UG students.


Module Leader:
BHCS2006 Infection, Immunity & Disease
BHCS2012 Infection Sciences in Health & Disease
BHCS3003 Clinical Microbiology
BHCS3015 Clinical Microbiology

Research Project Supervisor:
BIOM5006 MSc Biomedical Sciences Research Projects (Biosensor and Microbiology focussed)

Other Teaching Involvement:
BHCS1006 Infection & Immunity
BHCS3016 Public Health Infection Sciences
BIOM5008 Clinical Microbiology

Research interests

Rapid point of care detection of Antimicrobial resistant (AMR) bacteria

By 2050 it is estimated that 10 million people world-wide will die as a result of being infected with drug- resistant bacteria (Lord Jim O’Neil, 2014). Bacteria which were previously susceptible to antibiotic treatment have evolved to develop resistance to commonly used antibiotics, rendering them ineffective to combat infection. Now there are fewer antibiotics available to treat patients with certain infections. Hence, there is a global drive not only to discover new antibiotics to combat these bacteria, but also to develop new types of diagnostics that can help diagnose infection at point of care.

My research focusses on designing rapid, point of care nucleic acid-based assays for detection of AMR resistance genes. This research is highly interdisciplinary in nature utilising microwave engineering (in collaboration with Cardiff University) to liberate bacterial/spore DNA and DNA electrochemistry within the assay itself. A key part of this research includes integration of the assay into novel label-free biosensors (in collaboration with Plymouth University) and understanding the mechanisms by which microwaves interact with the bacterial DNA. 

I have 10 years of experience in development of novel microwave enhanced rapid diagnostics for microbial detection.

INTERVIEW on my research (Royal Society of Biology): https://www.youtube.com/watch?v=jC9Qy_o8e3U

Areas of expertise associated with the above include: Field effect transistors, thin-film transistors, Microwave engineering, aptamer design, aptamer-functionalisation of nanoparticles, microwave-accelerated metal enhanced fluorescence, microbial bioinformatics and biosensor development.

C. difficile Infection control:

Clostridium difficile is the primary cause of hospital acquired infection globally. In the US it contributes to 14 000 deaths/year, costing ~$1 billion annually (CDC, USA), while in the UK between 2006-2011 it was responsible for 29 425 deaths, (Office for National Statistics) costing £500 million/year. Its spores are resistant to desiccation and microbicide treatment and are able to persist on surfaces for months. My research focusses on understanding how C. difficile spores adhere to varying clinical surfaces and respond to biocidal insult, as well as using next generation sequencing techniques to understand spore epidemiology.

 Antimicrobial Peptides

I have an ongoing interest in the discovery and activity of novel antimicrobial peptides from invertebrates.

Research degrees awarded to supervised students

I have independently supervised both undergraduate and postgraduate students.

Grants & contracts

Adherence of C. difficile spores to surgical gowns 
Dr. Robert Burky & Rideout Hospital, California, USA, & University of California (UCLA) 

December 2016
Total Value: £4143 

Real time, microwave enhanced detection (~5 minutes) of genomic Bacillus anthracis 
Cardiff School of Pharmacy and Pharmaceutical Sciences Travel Grant. Biology of Anthrax, Tampa, Florida. November 2016
Total Value: £1000

Adherence of C. difficile spores to surgical gowns 
Society for Applied Microbiology "Students into Work Grant" Miss Calie Dyer
June 2016
Total Value: £2500

Rapid detection of Bacillus anthracis 
Royal Mail. Co-I with Prof Les Baillie (PI)
October 2015-April 2016
Total Value: £8000

Bursting superbugs using caterpillars and microwaves
Cardiff University School of Engineering. Da Vinci Innovation Research Award
November 2014
Total Value: £3000
Microwave enhanced bedside detection of Clostridium difficile
Cardiff University IP Advisory Fund. Co-I with Prof Les Baillie (PI), Prof Adrian Porch and Dr. Jonathan Lees
June 2014-June 2015
Total Value: £69356

Microwave enhanced bedside detection of Clostridium difficile
Society for Applied Microbiology Presidents Fund. ClostPath, Palm Cove, Tropical North Queensland, Australia.
October 2013
Total Value: £700

Microwave enhanced bedside detection of Clostridium difficile
EPSRC Impact Acceleration Award. Co-I with Prof Les Baillie (PI), Prof Adrian Porch and Dr. Jonathan Lees
July 2013-September 2014
Total Value: £48000

Manduca sexta as a source of novel antimicrobial peptides 
Society for Applied Microbiology "Students into Work Grant" Mr Garyn Pritchard
June 2013
Total Value: £2500

Characterisation of the spores of C. difficile 
Society for Applied Microbiology Presidents Fund. International C. difficile symposium, Bled, Slovenia. 
September 2010
Total Value: £1000

Characterisation of the spores of C. difficile 
Marie Curie Actions Grant. ClostPath, Rome, Italy. 
October 2009
Total Value: £1000

Key publications are highlighted

Journals
Articles
Joshi LT, Welsch A, Hawkins J & Baillie L 2017 'The effect of hospital biocide sodium dichloroisocyanurate on the viability and properties of Clostridium difficile spores' Letters in Applied Microbiology , DOI PEARL
Imtiaz A, Lees J, Choi H & Joshi LT 2015 'An Integrated Continuous Class-<formula formulatype="inline"><tex Notation="TeX">${\rm F} ^{-1}$</tex></formula> Mode Power Amplifier Design Approach for Microwave Enhanced Portable Diagnostic Applications' IEEE Transactions on Microwave Theory and Techniques 63, (10) 3007-3015 , DOI PEARL
Joshi LT, Mali BL, Geddes CD & Baillie L 2014 'Extraction and Sensitive Detection of Toxins A and B from the Human Pathogen Clostridium difficile in 40 Seconds Using Microwave-Accelerated Metal-Enhanced Fluorescence' PLoS ONE 9, (8) e104334-e104334 , DOI PEARL
Joshi LT, Phillips DS, Williams CF, Alyousef A & Baillie L 2012 'Contribution of Spores to the Ability of Clostridium difficile To Adhere to Surfaces' Applied and Environmental Microbiology 78, (21) 7671-7679 , DOI PEARL
Drevinek P, Baldwin A, Lindenburg L, Joshi LT, Marchbank A, Vosahlikova S, Dowson CG & Mahenthiralingam E 2010 'Oxidative Stress of Burkholderia cenocepacia Induces Insertion Sequence-Mediated Genomic Rearrangements That Interfere with Macrorestriction-Based Genotyping' Journal of Clinical Microbiology 48, (1) 34-40 , DOI PEARL

Other academic activities

Patents (x2):

Baillie, L & Joshi L.T. PCT/GB2013/051149 & PCT/GB2013/051151. A screening method for the detection of Clostridium difficile.

Public Engagement:

New Scientist Live! “Ask a Biologist” Expert. “Could microwaves save my life?”. Royal Society of Biology. Excel Arena, London. Saturday 30th September 2017

Science and the Assembly- Antimicrobial Resistance. Invited Expert Panel Member. Detection of Antimicrobial Resistance. Now and the Future. National Assembly of Wales, Royal Society of Chemistry, 6th June 2017

STEM Event at Pontypridd High School 20th June 2016

“RS Accessing the Scientists” event at Houses of Parliament, Westminster, UK (23rd November 2015).

Stem Live! Promoting Engineering to KS3 school pupils. Cardiff University 16th September 2015.

BBC Radio Wales interview about “Women in Science” June 2015.

“SoapBox Science Swansea June 2015” promoting and disseminating research done by female scientists in STEM. Given to Public. “Microwaving superbugs – transforming point-of-care diagnostics” & BBC Radio Wales interview

SET for Britain Finalist March 2015. Houses of Parliament, Westminster, UK.

Broadcast on Radio Cardiff December 2014 to explain my research into rapid detection of AMR

Institute of Engineering Schools Christmas Lecture December 2014 “Bug busting- new microwave assisted approaches for detecting antibiotic resistant bacteria”. Given to pupils from high schools in the local area to encourage interest in STEM subjects.

Environment Day 2014:28th October, St Davids Centre: honey as a source of new medicines.

“Biology Rocks” at National Museum, Cardiff, 2014; encouraging children at primary school level to learn about antibiotic resistant bacteria

Cardiff Science Festival 2014: Institute of Engineering and Technology (IET) Public Lecture,Cardiff Science Festival July 2014. “Bug busting- new microwave assisted approaches for detecting antibiotic resistant bacteria”. Informing public about antimicrobial resistance.



Additional information

I am a strong advocate for promoting STEM with the aim of encouraging interest in studying these subjects.
I continue to mentor students with their prospective scientific career choices and am heavily involved in varying Women in Science mentorship schemes. I am interested in advancing equality across HE, specifically with regard to Race Equality (Race Equality Charter) and Gender Equality through the Athena Swan Charter at the University of Plymouth.

I am part of:
Inspiring the Future
STEM Ambassador
STEM Plymouth
Women in STEM Plymouth

Links

https://www.researchgate.net/profile/Lovleen_Joshi