Professor Neil Avent
Profiles

Professor Neil Avent

Professor of Molecular Diagnostics and Transfusion Medicine

School of Biomedical & Healthcare Sciences (Plymouth University Peninsula Schools of Medicine and Dentistry)

Role

Head of School, Biomedical and Healthcare Sciences

Qualifications

BSc Bristol Polytechnic, Upper second class, Applied Biological sciences, 1984.
PhD University of Bristol, Department of Biochemistry, 1989.
PGCertHE UWE, Bristol, 2001.


Full Biog http://uk.linkedin.com/pub/neil-avent/8/a02/480

PA Ms Sara Wing
Sara.wing@plymouth.ac.uk

Selected recent presentations have been added to "publications" section

Professional membership

British Blood Transfusion Society
European Hematology Association
British Society for Proteome Research

Roles on external bodies

Member Transfusion Medicine Advisory Board, Grifols SA.

Associate editor : Frontiers in Gynecology and Obstetrics

Staff serving as external examiners

University of Chester,
Biomedical sciences programme 2005-2009

External examiner for PhDs, Universities of Aberdeen, Chester, Strathclyde, Warwick, UWE, Bristol and Portsmouth.

Research interests

Avent lab current Research activity

 

1.     Free fetal DNA (ffDNA) in maternal plasma (Dr Tracey Madgett, Kelly Sillence, Hannah Thompson)

ffDNA represents the greatest potential for delivering routine non-invasive prenatal diagnosis, and especially coupled with next-generation sequencing (NGS) technologies could easily become the norm for the diagnosis of aneuploidy. Unfortunately  NGS technology presently is prohibitively expensive, and we have developed methods for the enrichment of fetal DNA and depletion of maternal DNA in maternal plasma samples. The study is set to exploit the digital PCR platform we have just acquired within the Plymouth post-genomics Centre, which is due to open in November 2011. The methods involve relatively straightforward manipulations of the PCR procedure to inhibit the amplification of maternal DNA, and in one assay to actively destroy it. The work is currently funded by the NIHR, and is in collaboration with Prof Lyn Chitty (ICH/UCL/GOSH).

2.     Analysis of fetal cells in maternal blood (Dr Michele Kiernan)

Despite the huge advances in the study of ffDNA (described above) there are distinct advantages in being able to work with single purified fetal cells. Based  on our previous work, we are isolating fetal erythroblasts using our FACSAria 2 flow cytometer, and investigation the transcriptome using next-generation sequencing. With this we hope to identify fetal erythroblast specific biomarkers to enable their more efficient isolation from maternal blood samples. We consider the lack of suitable biomarkers of erythroid cells to be a key factor in the ineffectiveness of fetal cell isolation methodologies in the past.

3.     Molecular Investigation of Mechanisms of red cell turnover (Dr Kris Jeremy)

Continuing a project started in the 2000s, we are studying the role of the red cell form of CD47 and how huge numbers of effete red cells are removed from the circulation on a daily basis. We are using proteomics techniques to achieve this and are using normal red cells and CD47 deficient cells. Post-translational modification (PTM), involving PKA and PKC phosphorylation of red cell membrane components are critical in this process, and we are studying the precise molecular pathway involved. We have recently obtained a Thermo orbitrap Velos elite mass spectrometer (the first such instrument in the UK) to investigate the nature of the PTMs involved. The study is in collaboration with Prof Rob Barker (University of Aberdeen) as is funded by the Wellcome trust.


4.     New Serum Screening biomarkers for aneuploidy (Kelly Sillence)

We have identified a significant number of biomarkers using CVS samples from Down and non-Down mothers by transcriptomics, and by previous proteomics studies (see Heywood et al., (2011) in publications list). We are validating these markers after raising monoclonal antibodies to them. Many Down-specific changes involve PTMs and we will use our new Orbitrap velos mass spectrometer to identify their molecular nature- knowing precisely which protein species is involved. Study funded by the Fetal medicine Foundation in collaboration with Prof Kypros Nicolaides and Dr Ranjit Akolekar

 

5.     New roles for the CD47 glycoprotein (Dr Kris Jeremy)

       A study in collaboration with Prof Oliver Hanemann (PCMD), we are investigating the binding partners of CD47 in neural tissue, and then using CD47 ligands as potential new therapeutic agents against brain tumours, especially Schannoma.

4.     Next generation sequencing for blood groups (Amr Halawani, Malik Altayar, Tracey Madgett, Michele Kiernan, Tony Reynolds)

We have developed methodology to sequence the entire genomic segments of all clinically significant blood groups. This work has been performed on an Ion-Torrent PGM sequencer. Preliminary analysis of the KEL, FY, RHCE and RHD genes has revealed a hitherto unrealised level of variation within both coding and non-coding sequences. We will develop this technology to be sufficiently robust for diagnostic use, and will use new NGS technologies as soon as they are available.



Research degrees awarded to supervised students

PhD completions

Jill Storry (2000) UWE, Bristol (Second Supervisor)

Wendy Liu (2001) University of Bristol (Director of Studies)

Kirstin Finning (2003) UWE, Bristol (Director of Studies)

Emma Court (2003) UWE, Bristol (Second supervisor)

Lucy Skinner (2004) UWE, Bristol (Director of Studies)

Charlotte Spink (2004) UWE, Bristol (Second supervisor)

Zoe Plummer (2006) UWE, Bristol (Director of Studies)

Lisa Spary (2007) UWE, Bristol (Second supervisor)

Amanda Wallington (2007) UWE, Bristol (Director of Studies)

Kevin Kemp (2007) UWE, Bristol (Second supervisor)

Kelly Sanders (2008) UWE, Bristol (Second supervisor)

Sina Mueller (2009) University of Goettingen (Second supervisor) MD

Alistair Kemp (2009) UWE, Bristol (Second supervisor)

Wei Yuan (2011) University of Bristol (Second Supervisor)

Charlene Porter (2011) University of Aberdeen (Second supervisor)

Kin Choi (2011) University of Bristol (Second Supervisor)

Kris Jeremy (2012) UWE, Bristol (Director of Studies)

Amr Halawani (2016) PUPSMD (Director of Studies)

Kelly Silence (2016) PUPSMD (Director of Studies)

Grants & contracts

Grants since 2003
(1)    EU Framework V quality of Life programme “Cell factory” 2003-2006. Blood Grouping and Genotyping: Improving patient safety and Blood Transfusion compatibility. UWE, Bristol lead partner (N.D. Avent consortium co-ordinator, partners in Germany, Netherlands, Sweden, Czech Republic and Spain) (BloodGen consortium) €2,350,000 (http://www.bloodgen.co.uk). (2)    National Blood Service 2003-2008 Establishment of the Erythrocyte Proteome and Blood Grouping using Molecular Phenotyping  £345,494 (with Prof Marion Scott, Bristol Institute for Transfusion Science) (3)        EU Framework VI Special Advances in Fetal Evaluation (SAFE) network of excellence 12 million €. European Union framework VI - call genomics in health.  2004-2009 In collaboration with 52 EU partners (Dr Sinuhe Hahn co-ordinator). NDA Workpackage 3 leader, and chair of the network steering committee . UWE allocation - first 18 months €159,600, 2nd 18 months €376,800 (60 month project total) 3rd 18 months . (http://www.safenoe.org/) (4)        Mother and Baby trust, UK High Throughput non-invasive Genotyping £3,059 (With Dr Debbie Maddocks and Prof Peter Soothill) March 2004 (5)        EU Framework VI STREP – NEST Adventure 2005-2008 Isolation of fetal cells from maternal blood a nanomolecular approach (SAFER)" UWE allocation  €320,000 (http://www.etseq.urv.es/dinamic/SAFER/ ) Project co-ordinator Dr Ciara O’Sullivan  

(6)  “Transcriptomics of Down syndrome placental samples” 2008-2009 N.D. Avent and D.G. Maddocks, Fetal Medicine foundation £45,000

(7) “RAPID” Start date 01 January 2009-2014 National Institute of Health Research programme grant (Prof Lyn Chitty co-ordinator) £2 million total funding, University of Plymouth allocation  £50,000

(8) EUROGENTEST 2 Jan-2011-Jan 2014, EC-FP7 Standardisation of genetic testing in Europe, Workpackage leader, Non-Invasive Prenatal Diagnosis, University of Plymouth funding  €145,000

Key publications are highlighted

Journals
Articles
Li B, Pan G, Suhail A, Islam K, Avent N & Davey P 2017 'Deep UV hardening of photoresist for shaping of graphene and lift-off fabrication of back-gated field effect biosensors by ion-milling and sputter deposition' Carbon 118, 43-49 , DOI PEARL
Sillence KA, Halawani AJ, Tounsi WA, Clarke KA, Kiernan M, Madgett TE & Avent ND 2017 'Rapid RHD Zygosity Determination Using Digital PCR' Clinical Chemistry Author Site , DOI PEARL
Li B, Pan G & Avent N 2016 'A Simple Approach to Preparation of Graphene/Reduced Graphene Oxide/Polyallylamine Electrode and Their Electrocatalysis for Hydrogen Peroxide Reduction' Journal of Nanoscience and Nanotechnology , DOI PEARL
Sillence KA, Roberts LA, Hollands HJ, Thompson HP, Kiernan M, Madgett TE, Ross Welch C & Avent ND 2015 'Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR' Clinical Chemistry 61, (11) 1399-1407 , DOI PEARL
Li B, Pan G, Avent ND, Lowry RB, Madgett TE & Waines PL 2015 'Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection' Biosensors and Bioelectronics 72, 313-319 , DOI PEARL
Li B, Pan G, Jamil NY, Al Taan L, Awan S & Avent N 2015 'Shielding technique for deposition of Au electrical contacts on graphene by sputtering' JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A 33, (3) Author Site , DOI PEARL
Alrammah H, Woldie WA, Avent N & Jackson SK 2014 'Overexpression of lysophosphatidylcholine acyltransferase 2 (LPCAT2) up-regulated LPS-induced responses in a murine macrophage' IMMUNOLOGY 143, 104-105 Author Site
Avent ND 2014 'Prenatal testing for hemolytic disease of the newborn and fetal neonatal alloimmune thrombocytopenia – current status' Expert Review of Hematology 7, (6) 741-745 , DOI
Avent ND 2013 'Glycophorin C ligation: another biochemical pathway in red blood cell senescence?' Transfusion 53, (10) Author Site , DOI
Avent ND 2013 'Maternal plasma biomarkers for down syndrome: present and future' Drugs Today (Barc) 49, (2) 145-152 Author Site , DOI
Sillence KA, Madgett TE, Roberts LA, Overton TG & Avent ND 2013 'Non-invasive screening tools for Down's Syndome: A review' Diagnostics 3, (2) 291-314 , DOI
Haer-Wigman L, Veldhuisen B, Jonkers R, Lodén M, Madgett TE, Avent ND, de Haas M & van der Schoot CE 2013 'RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification' Transfusion 53, (7) 1559-1574 Author Site , DOI
Heywood W, Mills K, Wang D, Hogg J, Madgett TE, Avent ND & Chitty LS 2012 'Identification of new biomarkers for Down's syndrome in maternal plasma' J Proteomics 75, (9) 2621-2628 Author Site , DOI
Webb A, Madgett TE, Miran T, Sillence K, Kaushik N, Kiernan M & Avent ND 2012 'NON INVASIVE PRENATAL DIAGNOSIS OF ANEUPLOIDY: NEXT GENERATION SEQUENCING OR FETAL DNA ENRICHMENT?' BALKAN JOURNAL OF MEDICAL GENETICS 15, 17-26 Author Site , DOI
Avent ND 2012 'Refining noninvasive prenatal diagnosis with single-molecule next-generation sequencing' Clin Chem 58, (4) 657-658 Author Site , DOI
Heywood W, Wang D, Madgett TE, Avent ND, Eaton S, Chitty LS & Mills K 2012 'The development of a peptide SRM-based tandem mass spectrometry assay for prenatal screening of Down syndrome' J Proteomics 75, (11) 3248-3257 Author Site , DOI
Heywood WE, Madgett TE, Wang D, Wallington A, Hogg J, Mills K & Avent ND 2011 '2D DIGE analysis of maternal plasma for potential biomarkers of Down Syndrome' Proteome Sci 9, Author Site , DOI
Attilakos G, Maddocks DG, Davies T, Hunt LP, Avent ND, Soothill PW & Grant SR 2011 'Quantification of free fetal DNA in multiple pregnancies and relationship with chorionicity' Prenat Diagn 31, (10) 967-972 Author Site , DOI
Diraison F, Hayward K, Sanders KL, Brozzi F, Lajus S, Hancock J, Francis JE, Ainscow E, Bommer UA & Molnar E 2011 'Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells' Diabetologia 54, (2) 368-379 Author Site , DOI
May JE, Morse R, Xu J, Avent ND, Cox C, Wexler SA & Donaldson C 2010 'An in vitro model of chemotherapeutic damage to mesenchymal stem cells' MUTAGENESIS 25, (6) 633-633 Author Site
Wigman L, Veldhuisen B, Soussan AA, Avent ND, Madgett TE, Maddocks DG, de Haas M & van der Schoot CE 2010 'DEVELOPMENT OF A MULTIPLEX LIGAND-DEPENDENT PROBE AMPLIFICATION (MLPA) ASSAY TO DETERMINE VARIANTS IN THE RHD AND RHCE GENE' VOX SANGUINIS 99, 506-506 Author Site
Jeremy KP, Plummer ZE, Head DJ, Madgett TE, Sanders KL, Wallington A, Storry JR, Gilsanz F, Delaunay J & Avent ND 2009 '4.1R-deficient human red blood cells have altered phosphatidylserine exposure pathways and are deficient in CD44 and CD47 glycoproteins' Haematologica 94, (10) 1354-1361 Author Site , DOI PEARL
Avent ND, Madgett TE, Maddocks DG & Soothill PW 2009 'Cell-free fetal DNA in the maternal serum and plasma: current and evolving applications' Curr Opin Obstet Gynecol 21, (2) 175-179 Author Site , DOI
Flegel WA, von Zabern I, Doescher A, Wagner FF, Strathmann KP, Geisen C, Palfi M, Písacka M, Poole J & Polin H 2009 'D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters' Transfusion 49, (6) 1059-1069 Author Site , DOI
Avent ND 2009 'Large-scale blood group genotyping: clinical implications' Br J Haematol 144, (1) 3-13 Author Site , DOI
Alberry MS, Maddocks DG, Hadi MA, Metawi H, Hunt LP, Abdel-Fattah SA, Avent ND & Soothill PW 2009 'Quantification of cell free fetal DNA in maternal plasma in normal pregnancies and in pregnancies with placental dysfunction' Am J Obstet Gynecol 200, (1) 98.e1-98.e6 Author Site , DOI
Avent ND, Martinez A, Flegel WA, Olsson ML, Scott ML, Nogués N, Písacka M, Daniels GL, Muñiz-Diaz E & Madgett TE 2009 'The Bloodgen Project of the European Union, 2003-2009' Transfus Med Hemother 36, (3) 162-167 Author Site , DOI
Maddocks DG, Alberry MS, Attilakos G, Madgett TE, Choi K, Soothill PW & Avent ND 2009 'The SAFE project: towards non-invasive prenatal diagnosis' Biochem Soc Trans 37, (Pt 2) 460-465 Author Site , DOI
May JE, Xu J, Morse HR, Avent ND & Donaldson C 2009 'Toxicity testing: the search for an in vitro alternative to animal testing' Br J Biomed Sci 66, (3) 160-165 Author Site
Avent ND 2009 'Transfusion medicine education session' Expert Rev Hematol 2, (6) 641-643 Author Site , DOI
Avent ND, Plummer ZE, Madgett TE, Maddocks DG & Soothill PW 2008 'Post-genomics studies and their application to non-invasive prenatal diagnosis' Semin Fetal Neonatal Med 13, (2) 91-98 Author Site , DOI
Zimmermann BG, Maddocks DG & Avent ND 2008 'Quantification of circulatory fetal DNA in the plasma of pregnant women' Methods Mol Biol 444, 219-229 Author Site , DOI
Avent ND 2008 'RHD genotyping from maternal plasma: guidelines and technical challenges' Methods Mol Biol 444, 185-201 Author Site , DOI
Chitty LS, van der Schoot CE, Hahn S & Avent ND 2008 'SAFE—TheSpecial Non-invasiveAdvances inFetal and NeonatalEvaluation Network: aims and achievements' Prenatal Diagnosis 28, (2) 83-88 , DOI
Avent ND 2008 'The Bloodgen Project of the European Union' PROGRESS AND CHALLENGES IN TRANSFUSION MEDICINE, HEMOSTASIS AND HEMOTHERAPY: STATE OF THE ART 2008 178-188 Author Site , DOI
Avent ND 2008 'Unraveling the immune response during AIHA' BLOOD 111, (2) 483-483 Author Site , DOI
Alberry M, Maddocks D, Jones M, Abdel Hadi M, Abdel-Fattah S, Avent N & Soothill PW 2007 'Free fetal DNA in maternal plasma in anembryonic pregnancies: confirmation that the origin is the trophoblast' Prenat Diagn 27, (5) 415-418 Author Site , DOI
Avent ND 2007 'Large scale blood group genotyping' Transfus Clin Biol 14, (1) 10-15 Author Site , DOI
Alberry MS, Illanis S, Maddocks DG, Fattah SA, Zimmermann BG, Avent ND & Soothill PW 2007 'Maternal levels of free fetal DNA are elevated in pregnancies with growth restriction due to placental dysfunction' SAJOG-SOUTH AFRICAN JOURNAL OF OBSTETRICS AND GYNAECOLOGY 13, (2) 60-63 Author Site
Avent ND 2007 'New insight into the Rh system: structure and function' ISBT SCIENCE SERIES, VOL 2, NO 1 2, (1) 35-43 Author Site , DOI
Avent ND, Martinez A, Flegel WA, Olsson ML, Scott ML, Nogués N, Písacka M, Daniels G, van der Schoot E & Muñiz-Diaz E 2007 'The BloodGen project: toward mass-scale comprehensive genotyping of blood donors in the European Union and beyond' Transfusion 47, (1 Suppl) 40S-46S Author Site , DOI
Avent ND, Madgett TE, Lee ZE, Head DJ, Maddocks DG & Skinner LH 2006 'Molecular biology of Rh proteins and relevance to molecular medicine' Expert Rev Mol Med 8, (13) 1-20 Author Site , DOI
Avent ND & Chitty LS 2006 'Non-invasive diagnosis of fetal sex; utilisation of free fetal DNA in maternal plasma and ultrasound' Prenat Diagn 26, (7) 598-603 Author Site , DOI
Zimmermann BG, Holzgreve W, Avent N & Hahn S 2006 'Optimized real-time quantitative PCR measurement of male fetal DNA in maternal plasma' Ann N Y Acad Sci 1075, 347-349 Author Site , DOI
Avent ND, Martinez A, Flegel W, Olsson M, Nogues N, Pisacka M, Scott M, Daniels G & de Haas M 2006 'The bloodgen project: Toward mass scale genotyping for human blood group antigens' VOX SANGUINIS 91, 33-34 Author Site
Illanes S, Avent N & Soothill PW 2005 'Cell-free fetal DNA in maternal plasma: an important advance to link fetal genetics to obstetric ultrasound' Ultrasound Obstet Gynecol 25, (4) 317-322 Author Site , DOI
Head DJ, Lee ZE, Poole J & Avent ND 2005 'Expression of phosphatidylserine (PS) on wild-type and Gerbich variant erythrocytes following glycophorin-C (GPC) ligation' Br J Haematol 129, (1) 130-137 Author Site , DOI
Avent ND 2005 'High variability of the RH locus in different ethnic backgrounds' Transfusion 45, (3) 293-294 Author Site , DOI
Head DJ, Lee ZE, Swallah MM & Avent ND 2005 'Ligation of CD47 mediates phosphatidylserine expression on erythrocytes and a concomitant loss of viability in vitro' Br J Haematol 130, (5) 788-790 Author Site , DOI
Conroy MJ, Bullough PA, Merrick M & Avent ND 2005 'Modelling the human rhesus proteins: implications for structure and function' Br J Haematol 131, (4) 543-551 Author Site , DOI
Bianchi DW, Avent ND, Costa J-M & van der Schoot CE 2005 'Noninvasive prenatal diagnosis of fetal Rhesus D: ready for Prime(r) Time' Obstet Gynecol 106, (4) 841-844 Author Site , DOI
Court EL, Smith MA, Avent ND, Hancock JT, Morgan LM, Gray AG & Smith JG 2004 'DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines' Leuk Res 28, (7) 743-753 Author Site , DOI
Finning KM, Martin PG, Soothill PW & Avent ND 2002 'Prediction of fetal D status from maternal plasma: introduction of a new noninvasive fetal RHD genotyping service' Transfusion 42, (8) 1079-1085 Author Site
Scott M, Voak D, Liu W, Jones JW & Avent ND 2002 'Using monoclonal antibodies and site directed mutagenesis to map the epitopes of the blood group Rh D antigen' EPITOPE RECOGNITION SINCE LANDSTEINER'S DISCOVERY 83-101 Author Site
Avent ND 2001 'A new chapter in Rh research: Rh proteins are ammonium transporters' Trends Mol Med 7, (3) 94-96 Author Site
Avent ND 2001 'Molecular biology of the Rh blood group system' J Pediatr Hematol Oncol 23, (6) 394-402 Author Site
Avent ND 2000 'Recombinant technology in transfusion medicine' Curr Pharm Biotechnol 1, (2) 117-135 Author Site
Avent ND, Liu W, Jones JW, Scott ML & Voak D 2000 'Response - Limitations of prediction of epitope structure from corresponding antibody sequence' BLOOD 96, (3) 1197-1199 Author Site
Avent ND, Liu W, Scott ML, Jones JW & Voak D 2000 'Site directed mutagenesis of the human Rh D antigen: molecular basis of D epitopes' Vox Sang 78 Suppl 2, 83-89 Author Site
Chang TY & Siegel DL 2000 'The limitations of site-directed mutagenesis in the localization of Rh D epitopes' Blood 96, (3) 1196-1199 Author Site
Singleton BK, Green CA, Avent ND, Martin PG, Smart E, Daka A, Narter-Olaga EG, Hawthorne LM & Daniels G 2000 'The presence of an RHD pseudogene containing a 37 base pair duplication and a nonsense mutation in africans with the Rh D-negative blood group phenotype' Blood 95, (1) 12-18 Author Site
Avent ND & Reid ME 2000 'The Rh blood group system: a review' Blood 95, (2) 375-387 Author Site
Liu W, Avent ND, Jones JW, Scott ML & Voak D 1999 'Molecular configuration of Rh D epitopes as defined by site-directed mutagenesis and expression of mutant Rh constructs in K562 erythroleukemia cells' Blood 94, (12) 3986-3996 Author Site
Liu W, Smythe JS, Scott ML, Jones JW, Voak D & Avent ND 1999 'Site-directed mutagenesis of the human D antigen: definition of D epitopes on the sixth external domain of the D protein expressed on K562 cells' Transfusion 39, (1) 17-25 Author Site
Avent ND 1999 'The rhesus blood group system: insights from recent advances in molecular biology' Transfus Med Rev 13, (4) 245-266 Author Site
Olsson ML, Hansson C, Avent ND, Akesson IE, Green CA & Daniels GL 1998 'A clinically applicable method for determining the three major alleles at the Duffy (FY) blood group locus using polymerase chain reaction with allele-specific primers' Transfusion 38, (2) 168-173 Author Site
Wiener E, Shiels A, Wickramasinghe SN & Avent ND 1998 'Effect of 1-beta-D-arabino-furanosyl-cytosine (ara-C) induction of K562 cells on expression of Rh and other blood group active proteins' Br J Haematol 103, (1) 259-267 Author Site
Olsson ML, Smythe JS, Hansson C, Poole J, Mallinson G, Jones J, Avent ND & Daniels G 1998 'The Fyx phenotype is associated with a missense mutation in the Fyb allele predicting Arg89Cys in the Duffy glycoprotein' British Journal of Haematology 103, (4) 1184-1191 , DOI
Daniels GL, Faas BH, Green CA, Smart E, Maaskant-van Wijk PA, Avent ND, Zondervan HA, von dem Borne AE & van der Schoot CE 1998 'The VS and V blood group polymorphisms in Africans: a serologic and molecular analysis' Transfusion 38, (10) 951-958 Author Site
Avent ND, Martin PG, ArmstrongFisher SS, Liu W, Finning KM, Maddocks D & Urbaniak SJ 1997 'Evidence of genetic diversity underlying Rh D-, weak D (D-u), and partial D phenotypes as determined by multiplex polymerase chain reaction analysis of the RHD gene' BLOOD 89, (7) 2568-2577 Author Site
Avent ND 1997 'Human erythrocyte antigen expression: its molecular bases' Br J Biomed Sci 54, (1) 16-37 Author Site
Avent ND, Liu W, Jones JW, Scott ML, Voak D, Pisacka M, Watt J & Fletcher A 1997 'Molecular analysis of Rh transcripts and polypeptides from individuals expressing the DVI variant phenotype: an RHD gene deletion event does not generate All DVIccEe phenotypes' Blood 89, (5) 1779-1786 Author Site
Avent ND, Jones JW, Liu W, Scott ML, Voak D, Flegel WA, Wagner FF & Green C 1997 'Molecular basis of the D variant phenotypes DNU and DII allows localization of critical amino acids required for expression of Rh D epitopes epD3, 4 and 9 to the sixth external domain of the Rh D protein' Br J Haematol 97, (2) 366-371 Author Site
Jones JW, Finning K, Mattock R, Williams M, Voak D, Scott ML & Avent ND 1997 'The serological profile and molecular basis of a new partial D phenotype, DHR' Vox Sang 73, (4) 252-256 Author Site
Scott ML, Voak D, Jones JW, Avent ND, Liu W, Hughes-Jones N & Sonneborn H 1996 'A structural model for 30 Rh D epitopes based on serological and DNA sequence data from partial D phenotypes' Transfus Clin Biol 3, (6) 391-396 Author Site
Smythe JS, Avent ND, Judson PA, Parsons SF, Martin PG & Anstee DJ 1996 'Expression of RHD and RHCE gene products using retroviral transduction of K562 cells establishes the molecular basis of Rh blood group antigens' Blood 87, (7) 2968-2973 Author Site
Avent ND, Liu W, Warner KM, Mawby WJ, Jones JW, Ridgwell K & Tanner MJ 1996 'Immunochemical analysis of the human erythrocyte Rh polypeptides' J Biol Chem 271, (24) 14233-14239 Author Site
Avent ND & Martin PG 1996 'Kell typing by allele-specific PCR (ASP)' Br J Haematol 93, (3) 728-730 Author Site
Avent ND, Finning KM, Liu W & Scott ML 1996 'Molecular biology of partial D phenotypes' Transfus Clin Biol 3, (6) 511-516 Author Site
DANIELS G, KING MJ, AVENT ND, KHALID G, REID M, MALLINSON G, SYMTHE J & CEDERGREN B 1993 'A POINT MUTATION IN THE GYPC GENE RESULTS IN THE EXPRESSION OF THE BLOOD GROUP-AN(A) ANTIGEN ON GLYCOPHORIN-D BUT NOT ON GLYCOPHORIN-C - FURTHER EVIDENCE THAT GLYCOPHORIN-D IS A PRODUCT OF THE GYPC GENE' BLOOD 82, (10) 3198-3203 Author Site
Huber PA, Redwood CS, Avent ND, Tanner MJ & Marston SB 1993 'Identification of functioning regulatory sites and a new myosin binding site in the C-terminal 288 amino acids of caldesmon expressed from a human clone' J Muscle Res Cell Motil 14, (4) 385-391 Author Site
CARRITT B, BLUNT T, AVENT N, DANIELS G & STEERS F 1993 'Rh null phenotypes are not due to a gross deletion and can occure on different Rh genetic backgrounds' Annals of Human Genetics 57, (4) 273-279 , DOI
MacGeoch C, Mitchell CJ, Carritt B, Avent ND, Ridgwell K, Tanner MJ & Spurr NK 1992 'Assignment of the chromosomal locus of the human 30-kDal Rh (rhesus) blood group-antigen-related protein (Rh30A) to chromosome region 1p36.13----p34' Cytogenet Cell Genet 59, (4) 261-263 Author Site
Ridgwell K, Spurr NK, Laguda B, MacGeoch C, Avent ND & Tanner MJ 1992 'Isolation of cDNA clones for a 50 kDa glycoprotein of the human erythrocyte membrane associated with Rh (rhesus) blood-group antigen expression' Biochem J 287 ( Pt 1), 223-228 Author Site
AVENT ND, BUTCHER SK, LIU W, MAWBY WJ, MALLINSON G, PARSONS SF, ANSTEE DJ & TANNER MJA 1992 'LOCALIZATION OF THE C-TERMINI OF THE RH (RHESUS) POLYPEPTIDES TO THE CYTOPLASMIC FACE OF THE HUMAN ERYTHROCYTE-MEMBRANE' JOURNAL OF BIOLOGICAL CHEMISTRY 267, (21) 15134-15139 Author Site
King MJ, Avent ND, Mallinson G & Reid ME 1992 'Point mutation in the glycophorin C gene results in the expression of the blood group antigen Dha' Vox Sang 63, (1) 56-58 Author Site
Avent ND, Ridgwell K, Tanner MJ & Anstee DJ 1990 'cDNA cloning of a 30 kDa erythrocyte membrane protein associated with Rh (Rhesus)-blood-group-antigen expression' Biochem J 271, (3) 821-825 Author Site
Mallinson G, Anstee DJ, Avent ND, Ridgwell K, Tanner MJ, Daniels GL, Tippett P & von dem Borne AE 1990 'Murine monoclonal antibody MB-2D10 recognizes Rh-related glycoproteins in the human red cell membrane' Transfusion 30, (3) 222-225 Author Site
Tanner MJ, Anstee DJ, Mallinson G, Ridgwell K, Martin PG, Avent ND & Parsons SF 1988 'Effect of endoglycosidase F-peptidyl N-glycosidase F preparations on the surface components of the human erythrocyte' Carbohydr Res 178, 203-212 Author Site
Avent N, Judson PA, Parsons SF, Mallinson G, Anstee DJ, Tanner MJ, Evans PR, Hodges E, Maciver AG & Holmes C 1988 'Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific' Biochem J 251, (2) 499-505 Author Site
Avent ND, Ridgwell K, Mawby WJ, Tanner MJ, Anstee DJ & Kumpel B 1988 'Protein-sequence studies on Rh-related polypeptides suggest the presence of at least two groups of proteins which associate in the human red-cell membrane' Biochem J 256, (3) 1043-1046 Author Site
Letters
Carritt B, Steers FJ & Avent ND 1994 'Prenatal determination of fetal RhD type' Lancet 344, (8916) 205-206 Author Site
Conference Papers
Barczyk M, Zhou L, Avent N & Hanemann O 2013 'Tumour suppressor merlin regulates thrombospondin 1 signalling via CD47' 381-381
Barczyk M, Zhou L, Avent N & Hanemann O 2012 'Tumour Suppressor Merlin Regulates Thrombospondin 1 Signalling Via CD47' 162-162
May J, Morse R, Xu J, Avent N, Febrey S, Cox C, Wexler S & Donaldson C 2011 'Chemotherapy-induced damage to mesenchymal stem cells: an in vitro model' S308-S308
May E, Xu J, Morse R, Avent N, Cox C, Wexler S & Donaldson C 2010 'MODELING CHEMOTHERAPEUTIC DAMAGE TO MESENCHYMAL STEM CELLS' 664-664
May J, Xu J, Morse R, Avent N, Cox C, Wexler S & Donaldson C 2009 'Mesenchymal stem cell response to chemotherapeutic damage' S163-S163
Williams EH, Varadi A, Avent N & McLeod J 2008 'Dendritic cells as biosensors for allergic sensitisation' 111-111
Jeremy P, Gilsanz F, Delaunay J & Avent N 2008 'OPPOSING EFFECTS OF PROTEIN KINASE A AND C CAUSES DIFFERENTIAL PHOSPHATIDYLSERINE EXPOSURE IN A CD47 RECEPTOR MEDIATED ERYTHROCYTE APOPTOTIC PATHWAY' 188-188
Jeremy KP, Delaunay J, Gilsanz F & Avent ND 2008 'Opposing effects of protein kinase A and C causes differential phosphatidylserine exposure in a CD47 receptor mediated erythrocyte apoptotic pathway' 13th Congress of the European Hematology Association, June 12-15, 2008 Publisher Site
Jeremy KP, Delaunay J, Gilsanz F & Avent ND 2007 'Proteomic and Biochemical Profiling of 4.1R deficient red blood cells' 12th Congress of the European Hematology Association, June 7-10, 2007 Ferrata Storti 342-343 Publisher Site
Jeremy K, Plummer Z, Head D, Delaunay J & Avent N 2007 'Proteomic and biochemical profiling of 4.1R deficient red blood cells' 342-343
Varadi A, Sanders K, Avent N & Molnar E 2006 'Analysis of the beta cell and insulin-containing vesicle proteome' 313-313
Avent ND, Plummer ZE, Head DJ & Huang C-H 2006 'CD47 Glycoprotein Interacts with p4.1R and p55 in the erythrocyte' 334A-334A
Merrick M, Javelle A, Durand A, Severi E, Thornton J, Avent ND, Conroy MJ & Bullough PA 2006 'The Escherichia coli AmtB protein as a model system for understanding ammonium transport by Amt and Rh proteins' France 97-102 , DOI
Avent ND 2005 'Immunology of the RhD antigen' 47-48
Madgett TE, Maddocks DG, Nygren A, Choi K & Avent ND 2005 'Rapid molecular genotyping for RHD using multiplex ligation-dependent probe amplification technology' 129A-129A
Court EL, Smith A, Smith G, Avent ND, Hancock JT & Morgan L 2001 'DNA microarray screening of activated genes in marrow from normal subjects, ANLL patients and in ANLL cell lines' 157B-157B
Scott ML, Voak D, Liu W, Jones JW & Avent ND 2000 'Epitopes on Rh proteins' England 117-120
Avent ND, Finning KM, Martin PG & Soothill PW 2000 'Prenatal determination of fetal blood group status' England 155-162
Avent ND 1998 'Antenatal genotyping of the blood groups of the fetus' 365-374
Avent ND 1998 'Antenatal genotyping of the blood groups of the fetus' England 365-374
Avent ND 1998 'Molecular genetic methods: principles and feasibility in transfusion medicine' England 275-284
Avent ND 1998 'Molecular genetic methods: Principles and feasibility in transfusion medicine' 275-284
Olsson ML, Hansson C, Akesson IE, Avent ND & Daniels GL 1997 'Detection of the common alleles at the Duffy blood group locus by allele-specific primer PCR' S405-S405
Daniels GL, Green CA, Avent ND, Smart E, Faas BHW & vanderSchoot CE 1997 'The VS and V red cell polymorphisms in black South Africans' S403-S403
Smythe JS, Avent ND, Judson PA, Parsons SF, Martin P & Anstee DJ 1995 'Expression of RHD and RHCE gene products in K562 cells establishes the molecular basis of RH blood group antigens' 1878-1878

Other academic activities

Consultancy

2006-2009
Consultant for Progenika Biopharma (Blood group genotyping)
2011-2015 Consultant for Grifols SA (Transfusion Medicine Advisory board)