Establishment and characterisation of human
alveolar macrophage like cell lines
Reference number: 17-18-013
Mode of attendance: Full or part time
Start date: 1 October 2018
Director of Studies: Dr Gyorgy Fejer
Second supervisor: Professor Simon Jackson
Third supervisor: Dr Ashwin Dhanda
Fourth supervisor: Dr Chris Scotton
Fifth supervisor: Dr Tina Joshi
The Faculty of Medicine and Dentistry are seeking to attract PhD candidates of outstanding ability to join their exciting and rapidly expanding programme of internationally-rated research. The Faculty are committed to research excellence with a recent £25 million investment in staff and facilities. Medical research in our faculty was ranked first for outputs in REF2014.
Applications are invited from suitably qualified graduates, 2:1 or above, and preferably a relevant MSc qualification. International students must also have an IELTS score of 7.0 or above (with no less than 6.5 in any element).
Lung alveolar macrophages (AMs) play key pathogenic roles in a large number of important human respiratory disorders including infectious diseases caused by bacterial, fungal and viral pathogens. AMs are best investigated using primary cells because the genetic background of transformed macrophage lines may not be well defined. However, the availability of organ-derived primary macrophages is extremely limited which makes the study of host-pathogen interactions involving AMs problematic. Recently, we developed an in vitro model of non-transformed mouse AM-like cells (MPI cells, Fejer et al, PNAS 2013). The main characteristics of macrophages are conserved in mammals, but there are also variances between different species which make the use of human cells necessary.
The aim of this studentship is to establish
continuously growing human AM-like cell lines by adapting our
existing methods in mice and to demonstrate the suitability of these cells for
biomedical research using various approaches of immunology and molecular
biology. The student will obtain
training in molecular biology, immunology, microbiology and bioinformatics
techniques and will work in our new state-of-the-art purpose built facility equiped
with modern core facilities for cell culture, imaging, next generation
sequencing, proteomic analysis, cell biology and immunology.
For further information regarding the project, please contact Dr Gyorgy Fejer. However, applications must be made in accordance with the details shown below.