Dr Martyn Gilpin
Profiles

Dr Martyn Gilpin

Lecturer in Molecular Genetics

School of Biomedical & Healthcare Sciences (Plymouth University Peninsula Schools of Medicine and Dentistry)

Role

Member of the School of Biomedical & Healthcare Sciences Safety Committee - Biological Agents & Genetic Modification Safety Advisor

Member of the Plymouth University Safety Committee (1996-)

Plymouth University Advisor on Genetic Modification (1996-)

Module Leader BIOM3010  Clinical Microbiology (HCS)

Module Leader BIOM3019  Clinical Microbiology (BMS)

Module Leader BIOM3025  Microbial Diseases (HBS)

Module Leader BIOM2010 Infection in Health & Disease (HCS)

Module Leader BIOM2020 Infection, Immunity & Disease (HBS & BMS)

Qualifications

Lecturer in Molecular Genetics, University of Plymouth (1990 - present)

NAB Research Fellow, Plymouth Polytechnic (1988-1990). Bacterial Diseases of fish.

Research Fellow, Dental Research Unit of the Hunterian Institute,  Royal College of Surgeons of England,  University of London. MRC funded (1984-87).  “Cloning & Characterisation of Genes for Sucrose Metabolism from the Streptococcus mutans group of Cariogenic Bacteria”

Ph.D in Microbial Genetics, University of Reading (1980 -1983).  Genetic Analysis of Natural Variation in Escherichi coli” SERC Studentship.

B.Sc  (Hons) in  Microbiology,  University of Surrey (1977 -1980) Medical Microbiology pathway.

Professional membership

Member of The European Melioidosis Group.

Roles on external bodies

External Advisor to the Plymouth Marine Laboratory /Marine Biological Association Genetic Manipulation Committee. ( 2000-)

External Examiner, Ph.D : University of Exeter. September 2012. 

Teaching interests

Microbiology, microbial genetics, molecular techniques, gene cloning, microbial biotechnology, microbial pathogenicity, DNA sequence analysis & bioinformatics.

Teaching on the following Plymouth University modules:

 

BIOM3006: Personal Research and Professional Practice (BMS)

BIOM3010: Clinical Microbiology (HCS)

BIOM3019: Clinical Microbiology (BMS)

BIOM3025: Microbial Diseases (HBS)

BIOL2003:  Methods in Human Biosciences (HBS)

BIOM2010: Infection in Health & Disease (HCS)

BIOM2019: Evidence-Based Practice in Biomedical Science (BMS)

BIOM2020: Infection, Immunity & Disease (HBS & BMS)

BIOM1006: Infection and Immunity (BMS, HBS & HCS))

BIOL1007  Skills in Biomedical Investigation and Experimentation (HBS)

 

Research interests

Molecular basis of the pathogenicity of bacterial pathogens. Molecular detection and typing systems for bacterial pathogens particularly with repect to the fish pathogen Renibacterium salmoninarum (Bacterial Kidney Disease), the human and animal pathogen Burkholderia pseudomallei (Mellioidosis) and the food poisoning agent Vibrio parahaemolyticus. More recent work has focused on the possible involvement of microbes in the development of human forms of diabetes. In particular:

1) The association  Burkholderia cepacia group organisms with  the development of diabetes in cystic fibrosis patients, and

2) The possibility that Escherichia coli  antigens show cross reactivity with human insulin producing beta-pancreatic cells, leading to possible autoimmune disease.

Research degrees awarded to supervised students

Delores M. Furones Ph.D (1990) “Characterisation of Virulence Factors in the Fish Pathogen Yersinia ruckerii”

T. Hilton Grayson Ph.D (1993) “Molecular Cloning & Characterisation of Potential Vaccine Antigens from Renibacterium salmoninarum”

Andrew J. Evenden Ph.D (1993) “The Use of Gene Cloning Techniques in the Study of the Fish Pathogen Renibacterium salmoninarum”

Bingmei Yang. Ph.D (1998) “Isolation & Characterisation of Immunoglobulin Gene Superfamily Molecules from Lower Vertebrates (Fish)”

Clare Davies : Ph.D (2001)“Molecular Characterisation of Burkholderia pseudomallei” 

Magdy E. Mahfouz : Ph.D (2001) “ Molecular Characterisation of a Two  Component Regulatory System from Burkholderia pseudomallei”

Sarah M.  Alexander : Ph.D (2002) “Molecular Differentiation of Renibacterium salmoninarum Isolates”

Michelle Stone : Ph.D (2004) ”The Use of Prebiotics to Improve Fish Health”  

Edele Chambers :  M.Phil (2005) “Development of methods to assess the prevalence Renibacterium salmoninarum in selected river systems in England and Wales”

 Aklak Miah Ph.D (2009) “Characterisation & Molecular Typing of Clinical & Environmental Isolates of Vibrio parahaemolyticus”

Fazal Hadi Ph.D  (2010) “The Physiological & Molecular Characteristics of Chemically induced Abiotic Stress Resistant Mutants of Cauliflower (Brassica oleracea var. botrytis)”

Raid B. Nisr Ph.D (2012) “Molecular Characterisation of the Interaction of Microbes with the Insulin Pathway”

Hadaf M. Kadhim Ph.D (2012) “Molecular Characterisation of Clinical and Environmental Isolates of Vibrio parahaemolyticus”

Nisr, R. B., Moody, A. J., & Gilpin, M. L. (2011). Molecular mimicries of linear epitopes between pancreatic glutamic acid decarboxylase (GAD65) and proteins from Escherichia coli. In IMMUNOLOGY Vol. 135 (pp. 140). Retrieved from http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000297507100422&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008

Nisr, R. B., Moody, A. J., & Gilpin, M. L. (2012). Screening microorganisms for insulin binding reveals binding by Burkholderia multivorans and Burkholderia cenocepacia and novel attachment of insulin to Aeromonas salmonicida via the A-layer.. FEMS Microbiol Lett, 328(2), 93-99. doi:10.1111/j.1574-6968.2011.02484.x

Nisr, R. B., Russell, M. A., Chrachri, A., Moody, A. J., & Gilpin, M. L. (2011). Effects of the microbial secondary metabolites pyrrolnitrin, phenazine and patulin on INS-1 rat pancreatic β-cells.. FEMS Immunol Med Microbiol, 63(2), 217-227. doi:10.1111/j.1574-695X.2011.00844.x

Kadhim, H. M., Miah, A., Munn, C. B., & Gilpin, M. L. (2012). Development of a polymerase chain reaction (PCR) test for the detection of virulent forms of Vibrio parahaemolyticus.. Eur J Clin Microbiol Infect Dis, 31(4), 431-439. doi:10.1007/s10096-011-1324-9

GRAYSON, T., EVENDEN, A., GILPIN, M., & MUNN, C. (1995). PRODUCTION OF A RENIBACTERIUM-SALMONINARUM HEMOLYSIN FUSION PROTEIN IN ESCHERICHIA-COLI K12. DISEASES OF AQUATIC ORGANISMS, 22(2), 153-156. doi:10.3354/dao022153

GRAYSON, T., EVENDEN, A., GILPIN, M., & MUNN, C. (1995). PRODUCTION OF THE MAJOR SOLUBLE-ANTIGEN OF RENIBACTERIUM-SALMONINARUM IN ESCHERICHIA-COLI K12. DISEASES OF AQUATIC ORGANISMS, 22(3), 227-231. doi:10.3354/dao022227

GRAYSON, T., BRUNO, D., EVENDEN, A., GILPIN, M., & MUNN, C. (1995). IRON ACQUISITION BY RENIBACTERIUM-SALMONINARUM - CONTRIBUTION OF IRON REDUCTASE. DISEASES OF AQUATIC ORGANISMS, 22(2), 157-162. doi:10.3354/dao022157

Grayson, T. H., John, R. J., Wadsworth, S., Greaves, K., Cox, D., Roper, J., . . . Harris, J. E. (1995). Immunization of Atlantic salmon against the salmon louse: Identification of antigens and effects on louse fecundity. In JOURNAL OF FISH BIOLOGY Vol. 47 (pp. 85-94). doi:10.1111/j.1095-8649.1995.tb06046.x

McIntosh, D., Flano, E., Grayson, T. H., Gilpin, M. L., Austin, B., & Villena, A. J. (1997). Production of putative virulence factors by Renibacterium salmoninarum grown in cell culture. MICROBIOLOGY-UK, 143, 3349-3356. Retrieved from http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=A1997YB28800029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008

Grayson, T. H., Atienzar, F. A., Alexander, S. M., Cooper, L. F., & Gilpin, M. L. (2000). Molecular diversity of Renibacterium salmoninarum isolates determined by randomly amplified polymorphic DNA analysis.. Appl Environ Microbiol, 66(1), 435-438. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10618262

Grayson, T. H., Evenden, A. J., Gilpin, M. L., Martin, K. L., & Munn, C. B. (1995). A gene from Renibacterium salmoninarum encoding a product which shows homology to bacterial zinc-metalloproteases.. Microbiology, 141 ( Pt 6), 1331-1341. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/7545509

Grayson, T. H., Cooper, L. F., Atienzar, F. A., Knowles, M. R., & Gilpin, M. L. (1999). Molecular differentiation of Renibacterium salmoninarum isolates from worldwide locations.. Appl Environ Microbiol, 65(3), 961-968. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10049848

Evenden, A. J., Gilpin, M. L., & Munn, C. B. (1990). The cloning and expression of a gene encoding haemolytic activity from the fish pathogen Renibacterium salmoninarum.. FEMS Microbiol Lett, 59(1-2), 31-34. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2276613

Grayson, T. H., Alexander, S. M., Cooper, L. F., & Gilpin, M. L. (2000). Renibacterium salmoninarum isolates from different sources possess two highly conserved copies of the rRNA operon .. Antonie Van Leeuwenhoek, 78(1), 51-61. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11016696

Furones, M. D., Gilpin, M. L., & Munn, C. B. (1993). Culture media for the differentiation of isolates of Yersinia ruckeri, based on detection of a virulence factor.. J Appl Bacteriol, 74(4), 360-366. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/8486541

Grayson, T. H., Cooper, L. F., Wrathmell, A. B., Roper, J., Evenden, A. J., & Gilpin, M. L. (2002). Host responses to Renibacterium salmoninarum and specific components of the pathogen reveal the mechanisms of immune suppression and activation. Immunology, 106(2), 273-283.

Alexander, S. M., Grayson, T. H., Chambers, E. M., Cooper, L. F., Barker, G. A., & Gilpin, M. L. (2001). Variation in the spacer regions separating tRNA genes in Renibacterium salmoninarum distinguishes recent clinical isolates from the same location. Journal of Clinical Microbiology, 39(1), 119-128.

Snow, M., Barja, J., Colquhoun, D., Cunningham, C., Devold, M., Dopazo, C., . . . Thiery, R. (2004). Development of a European resource on the origins of pathogens of aquaculture: The Europa Project. Bulletin of the European Association of Fish Pathologists, 24(1), 54-57.

Grayson, T. H., Gilpin, M. L., Evenden, A. J., & Munn, C. B. (2001). Evidence for the immune recognition of two haemolysins of Renibacterium salmoninarum by fish displaying clinical symptoms of bacterial kidney disease (BKD). Fish & Shellfish Immunology, 11(4), 367-370.

Mahfouz, M. E., Grayson, T. H., Dance, D. A. B., & Gilpin, M. L. (2006). Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system. Bmc Microbiology, 6.