Dr Edwin Lasonder

Dr Edwin Lasonder

Associate Professor in Proteomics

School of Biomedical Sciences (Faculty of Medicine and Dentistry)


Associate Professor in Proteomics

Admissions Tutor MSc Biomedical Sciences


PhD Medical Sciences, University of Goningen, The Netherlands (1995)

Drs Organic Chemistry, University of Groningen, The Netherlands (1990)

Professional membership

- European Virtual Institute of Malaria Research


- Malsig 


Teaching interests

Proteomics Seminars  (undergraduate and postgraduate students) and Proteomics Workshops (postgraduate students)

Research interests

 My general research interest is the application of massspectrometry–based proteomics in biomedical research with a particular interestfor the human diseases malaria and cancer.

 The most severe form of malaria is caused by the infection of the parasite Plasmodium falciparum when an infected Anopheles mosquito takes a blood meal. Currently, about half of the 5500 P.falciparum genes are not functionally annotated due to poor homology with other species.  We apply proteome mining and proteome expression profiling for creating a proteomic catalogue of proteins expressed in all of the life cycle stages.  So far,we have characterised about 2600 P.fal proteins across seven developmental life cycle stages in human and mosquito hosts. The most recent work is focussed on establishing the parasite phosphoproteome in infected red blood cells(schizonts, merozoites) to decipher parasite-specific signalling pathways that allow development of new anti-malarial drugs.

Current projects

  1. The identification of the P.falciparum signalling pathways and mechanisms of red blood cell invasion and motility by a comparative phosphoproteome analysis of merozoites schizonts. Collaborators: dr A. Holder (MRC, London), dr G.Langsley (Institut Cochin, Paris, France), dr P.Alano (ISS, Rome, Italy)
  2. The P. falciparum proteome of male and female gametocytes. Collaborators: Dr. B. van Schaijk, prof dr R. Sauerwein (Radboud University Medical Centre, Nijmegen, The Netherlands)
  3. The identification of the signalling pathways that cause accelerated aging of red blood cells through infection of P. falciparum. Collaborators:dr G.Langsley (Institut Cochin, Paris, France)
  4. Dendriomics of Human DCs Cells Subsets; an integrated analysis of the transcriptome and proteome of human dendritic cells in vivo. Collaborators:K. Worah, dr S. Buschow and prof.dr. C.G. Figdor (Radboud University Medical Centre, Nijmegen, The Netherlands)
  5. Identifying common therapeutic targets in schwannomas and meningiomas brain tumours. Collaborators: K. Bassiri and prof dr. O.Hanemann  (Peninsula Medical School, Plymouth University)

Grants & contracts

- European Virtual Institute of Malaria Research


Key publications are highlighted

Dunn J, Ferluga S, Sharma V, Futschik M, Hilton DA, Adams CL, Lasonder E & Hanemann CO 2018 'Proteomic analysis discovers the differential expression of novel proteins and phosphoproteins in meningioma including NEK9, HK2 and SET and deregulation of RNA metabolism' EBioMedicine Author Site , DOI PEARL
Stone WJR, Campo JJ, Ouédraogo AL, Meerstein-Kessel L, Morlais I, Da D, Cohuet A, Nsango S, Sutherland CJ 2018 'Publisher Correction: Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity' Nature Communications 9, (1) , DOI PEARL
Stone WJR, Campo JJ, Ouédraogo AL, Meerstein-Kessel L, Morlais I, Da D, Cohuet A, Nsango S, Sutherland CJ 2018 'Unravelling the immune signature of Plasmodium falciparum transmission reducing immunity' Nature Communications , DOI PEARL
Tremp AZ, Sharma V, Carter V, Lasonder E & Dessens JT 2017 'LCCL protein complex formation in Plasmodium is critically dependent on LAP1' Mol Biochem Parasitol Author Site , DOI PEARL
Lasonder E 2017 'Clinical Proteomics: From Biological Sample to Clinical Exploitation' Proteomes 5, 10-12 , DOI PEARL
Bassiri K, Ferluga S, Sharma V, Syed N, Adams CL, Lasonder E & Hanemann CO 2017 'Global Proteome and Phospho-proteome Analysis of Merlin-deficient Meningioma and Schwannoma Identifies PDLIM2 as a Novel Therapeutic Target' EBioMedicine , DOI PEARL
Worah K, Mathan TS, Vu Manh TP, Keerthikumar S, Schreibelt G, Tel J, Duiveman-de Boer T, Sköld AE, van Spriel AB 2016 'Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function' Cell Rep 16, (11) 2953-2966 Author Site , DOI PEARL
Suárez-Cortes P, Sharma V, Bertuccini L, Costa G, Bannerman NL, Sannella AR, Williamson K, Klemba M, Levashina EA 2016 'Comparative proteomics and functional analysis reveal a role of P. falciparum osmiophilic bodies in malaria parasite transmission' Molecular & cellular proteomics : MCP PEARL
Middelbeek J, Vrenken K, Visser D, Lasonder E, Koster J, Jalink K, Clark K & van Leeuwen FN 2016 'The TRPM7 interactome defines a cytoskeletal complex linked to neuroblastoma progression' European Journal of Cell Biology , DOI PEARL
Lasonder E, Rijpma SR, van Schaijk BC, Hoeijmakers WA, Kensche PR, Gresnigt MS, Italiaander A, Vos MW, Woestenenk R 2016 'Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression' Nucleic acids research PEARL
Eich C, Lasonder E, Cruz LJ, Reinieren-Beeren I, Cambi A, Figdor CG & Buschow SI 2016 'Proteome Based Construction of the Lymphocyte Function-Associated Antigen 1 (LFA-1) Interactome in Human Dendritic Cells' PLOS ONE 11, (2) e0149637-e0149637 , DOI PEARL
Bouyer G, Reininger L, Ramdani G, D Phillips L, Sharma V, Egee S, Langsley G & Lasonder E 2016 'Plasmodium falciparum infection induces dynamic changes in the erythrocyte phospho-proteome' Blood cells, molecules & diseases 58, 35-44 PEARL
Zhou L, Lyons-Rimmer J, Ammoun S, Müller J, Lasonder E, Sharma V, Ercolano E, Hilton D, Taiwo I 2015 'The scaffold protein KSR1, a novel therapeutic target for the treatment of Merlin-deficient tumors' Oncogene 35, (26) 3443-3453 , DOI PEARL
Lasonder E, Green JL, Grainger M, Langsley G & Holder AA 2015 'Extensive differential protein phosphorylation as intraerythrocytic Plasmodium falciparum schizonts develop into extracellular invasive merozoites' Proteomics 15, (15) 2716-2729 Author Site , DOI
Müller PJ, Rigbolt KTG, Paterok D, Piehler J, Vanselow J, Lasonder E, Andersen JS, Schaper F & Sobota RM 2013 'Protein tyrosine phosphatase SHP2/PTPN11 mistargeting as a consequence of SH2-domain point mutations associated with Noonan Syndrome and leukemia' J Proteomics 84, 132-147 Author Site , DOI
Simon N, Lasonder E, Scheuermayer M, Kuehn A, Tews S, Fischer R, Zipfel PF, Skerka C & Pradel G 2013 'Malaria parasites co-opt human factor H to prevent complement-mediated lysis in the mosquito midgut' Cell Host Microbe 13, (1) 29-41 Author Site , DOI
Bosman GJCGM, Lasonder E, Groenen-Döpp YAM, Willekens FLA & Werre JM 2012 'The proteome of erythrocyte-derived microparticles from plasma: new clues for erythrocyte aging and vesiculation' J Proteomics 76 Spec No., 203-210 Author Site , DOI
Lasonder E, Green JL, Camarda G, Talabani H, Holder AA, Langsley G & Alano P 2012 'The Plasmodium falciparum schizont phosphoproteome reveals extensive phosphatidylinositol and cAMP-protein kinase A signaling' J Proteome Res 11, (11) 5323-5337 Author Site , DOI
Lasonder E, Treeck M, Alam M & Tobin AB 2012 'Insights into the Plasmodium falciparum schizont phospho-proteome' Microbes Infect 14, (10) 811-819 Author Site , DOI
Farhoud MH, Nijtmans LG, Wanders RJA, Wessels HJCT, Lasonder E, Janssen AJM, Rodenburg RRJ, van den Heuvel LP & Smeitink JAM 2012 'Impaired ubiquitin-proteasome-mediated PGC-1α protein turnover and induced mitochondrial biogenesis secondary to complex-I deficiency' Proteomics 12, (9) 1349-1362 Author Site , DOI
Buschow SI, Lasonder E, Szklarczyk R, Oud MM, de Vries IJM & Figdor CG 2012 'Unraveling the human dendritic cell phagosome proteome by organellar enrichment ranking' J Proteomics 75, (5) 1547-1562 Author Site , DOI
Szklarczyk R, Wanschers BF, Cuypers TD, Esseling JJ, Riemersma M, van den Brand MA, Gloerich J, Lasonder E, van den Heuvel LP 2012 'Iterative orthology prediction uncovers new mitochondrial proteins and identifies C12orf62 as the human ortholog of COX14, a protein involved in the assembly of cytochrome c oxidase' Genome Biol 13, (2) Author Site , DOI PEARL
Laurentino EC, Taylor S, Mair GR, Lasonder E, Bartfai R, Stunnenberg HG, Kroeze H, Ramesar J, Franke-Fayard B 2011 'Experimentally controlled downregulation of the histone chaperone FACT in Plasmodium berghei reveals that it is critical to male gamete fertility' Cell Microbiol 13, (12) 1956-1974 Author Site , DOI PEARL
De Franceschi L, Tomelleri C, Matte A, Brunati AM, Bovee-Geurts PH, Bertoldi M, Lasonder E, Tibaldi E, Danek A 2011 'Erythrocyte membrane changes of chorea-acanthocytosis are the result of altered Lyn kinase activity' Blood 118, (20) 5652-5663 Author Site , DOI
Silvestrini F, Lasonder E, Olivieri A, Camarda G, van Schaijk B, Sanchez M, Younis Younis S, Sauerwein R & Alano P 2010 'Protein export marks the early phase of gametocytogenesis of the human malaria parasite Plasmodium falciparum' Mol Cell Proteomics 9, (7) 1437-1448 Author Site , DOI
van der Voort R, Verweij V, de Witte TM, Lasonder E, Adema GJ & Dolstra H 2010 'An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells' J Leukoc Biol 87, (6) 1029-1039 Author Site , DOI
Buschow SI, Lasonder E, van Deutekom HWM, Oud MM, Beltrame L, Huynen MA, de Vries IJM, Figdor CG & Cavalieri D 2010 'Dominant processes during human dendritic cell maturation revealed by integration of proteome and transcriptome at the pathway level' J Proteome Res 9, (4) 1727-1737 Author Site , DOI
Mair GR, Lasonder E, Garver LS, Franke-Fayard BMD, Carret CK, Wiegant JCAG, Dirks RW, Dimopoulos G, Janse CJ 2010 'Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development' PLoS Pathog 6, (2) Author Site , DOI PEARL
Bosman GJCGM, Lasonder E, Groenen-Döpp YAM, Willekens FLA, Werre JM & Novotný VMJ 2010 'Comparative proteomics of erythrocyte aging in vivo and in vitro' J Proteomics 73, (3) 396-402 Author Site , DOI
Doerig C, Baker D, Billker O, Blackman MJ, Chitnis C, Dhar Kumar S, Heussler V, Holder AA, Kocken C 2009 'Signalling in malaria parasites. The MALSIG consortium' Parasite 16, (3) 169-182 Author Site , DOI
Lasonder E, Janse CJ, van Gemert G-J, Mair GR, Vermunt AMW, Douradinha BG, van Noort V, Huynen MA, Luty AJF 2008 'Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity' PLoS Pathog 4, (10) Author Site , DOI PEARL
Clark K, Middelbeek J, Dorovkov MV, Figdor CG, Ryazanov AG, Lasonder E & van Leeuwen FN 2008 'The α-kinases TRPM6 and TRPM7, but not eEF-2 kinase, phosphorylate the assembly domain of myosin IIA, IIB and IIC' FEBS Letters 582, (20) 2993-2997 , DOI
Bosman GJCGM, Lasonder E, Luten M, Roerdinkholder-Stoelwinder B, Novotný VMJ, Bos H & De Grip WJ 2008 'The proteome of red cell membranes and vesicles during storage in blood bank conditions' Transfusion 48, (5) 827-835 Author Site , DOI
Clark K, Middelbeek J, Lasonder E, Dulyaninova NG, Morrice NA, Ryazanov AG, Bresnick AR, Figdor CG & van Leeuwen FN 2008 'TRPM7 regulates myosin IIA filament stability and protein localization by heavy chain phosphorylation' J Mol Biol 378, (4) 790-803 Author Site , DOI
Clark K, Middelbeek J, Morrice NA, Figdor CG, Lasonder E & van Leeuwen FN 2008 'Massive autophosphorylation of the Ser/Thr-rich domain controls protein kinase activity of TRPM6 and TRPM7' PLoS One 3, (3) Author Site , DOI PEARL
Pandithage R, Lilischkis R, Harting K, Wolf A, Jedamzik B, Lüscher-Firzlaff J, Vervoorts J, Lasonder E, Kremmer E 2008 'The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility' J Cell Biol 180, (5) 915-929 Author Site , DOI PEARL
Cao G, Thébault S, van der Wijst J, van der Kemp A, Lasonder E, Bindels RJM & Hoenderop JGJ 2008 'RACK1 Inhibits TRPM6 Activity via Phosphorylation of the Fused α-Kinase Domain' Current Biology 18, (3) 168-176 , DOI
Outchkourov N, Vermunt A, Jansen J, Kaan A, Roeffen W, Teelen K, Lasonder E, Braks A, van de Vegte-Bolmer M 2007 'Epitope analysis of the malaria surface antigen pfs48/45 identifies a subdomain that elicits transmission blocking antibodies' J Biol Chem 282, (23) 17148-17156 Author Site , DOI
Tjalsma H, Lasonder E, Schöller-Guinard M & Swinkels DW 2007 'Shotgun immunoproteomics to identify disease-associated bacterial antigens: Application to human colon cancer' PROTEOMICS – Clinical Applications 1, (4) 429-434 , DOI
Tjalsma H, Schöller-Guinard M, Lasonder E, Ruers TJ, Willems HL & Swinkels DW 2006 'Profiling the humoral immune response in colon cancer patients: diagnostic antigens from Streptococcus bovis' Int J Cancer 119, (9) 2127-2135 Author Site , DOI
Vermeulen M, Walter W, Le Guezennec X, Kim J, Edayathumangalam RS, Lasonder E, Luger K, Roeder RG, Logie C 2006 'A feed-forward repression mechanism anchors the Sin3/histone deacetylase and N-CoR/SMRT corepressors on chromatin' Mol Cell Biol 26, (14) 5226-5236 Author Site , DOI
Le Guezennec X, Vermeulen M, Brinkman AB, Hoeijmakers WAM, Cohen A, Lasonder E & Stunnenberg HG 2006 'MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties' Mol Cell Biol 26, (3) 843-851 Author Site , DOI
Ozdemir A, Spicuglia S, Lasonder E, Vermeulen M, Campsteijn C, Stunnenberg HG & Logie C 2005 'Characterization of lysine 56 of histone H3 as an acetylation site in Saccharomyces cerevisiae' J Biol Chem 280, (28) 25949-25952 Author Site , DOI
Khan SM, Franke-Fayard B, Mair GR, Lasonder E, Janse CJ, Mann M & Waters AP 2005 'Proteome analysis of separated male and female gametocytes reveals novel sex-specific Plasmodium biology' Cell 121, (5) 675-687 Author Site , DOI
Vermeulen M, Carrozza MJ, Lasonder E, Workman JL, Logie C & Stunnenberg HG 2004 'In vitro targeting reveals intrinsic histone tail specificity of the Sin3/histone deacetylase and N-CoR/SMRT corepressor complexes' Mol Cell Biol 24, (6) 2364-2372 Author Site
van Ingen H, Lasonder E, Jansen JFA, Kaan AM, Spronk CAEM, Stunnenberg HG & Vuister GW 2004 'Extension of the Binding Motif of the Sin3 Interacting Domain of the Mad Family Proteins † , ‡' Biochemistry 43, (1) 46-54 , DOI
Lasonder E, Ishihama Y, Andersen JS, Vermunt AMW, Pain A, Sauerwein RW, Eling WMC, Hall N, Waters AP 2002 'Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry' Nature 419, (6906) 537-542 Author Site , DOI
Kloks CPAM, Spronk CAEM, Lasonder E, Hoffmann A, Vuister GW, Grzesiek S & Hilbers CW 2002 'The solution structure and DNA-binding properties of the cold-shock domain of the human Y-box protein YB-1' J Mol Biol 316, (2) 317-326 Author Site , DOI
Lasonder E, Schellekens GA, Koedijk DG, Damhof RA, Welling-Wester S, Feijlbrief M, Scheffer AJ & Welling GW 1996 'Kinetic analysis of synthetic analogues of linear-epitope peptides of glycoprotein D of herpes simplex virus type 1 by surface plasmon resonance' Eur J Biochem 240, (1) 209-214 Author Site
Bakker R, Lasonder E & Bos NA 1995 'Measurement of affinity in serum samples of antigen-free, germ-free and conventional mice after hyperimmunization with 2,4-dinitrophenyl keyhole limpet hemocyanin, using surface plasmon resonance' Eur J Immunol 25, (6) 1680-1686 Author Site , DOI
Schellekens GA, Lasonder E, Feijlbrief M, Koedijk DG, Drijfhout JW, Scheffer AJ, Welling-Wester S & Welling GW 1994 'Identification of the core residues of the epitope of a monoclonal antibody raised against glycoprotein D of herpes simplex virus type 1 by screening of a random peptide library' Eur J Immunol 24, (12) 3188-3193 Author Site , DOI
Lasonder E, Bloemhoff W & Welling GW 1994 'Interaction of lysozyme with synthetic anti-lysozyme D1.3 antibody fragments studied by affinity chromatography and surface plasmon resonance' J Chromatogr A 676, (1) 91-98 Author Site , DOI
Kostiainen R, Lasonder E, Bloemhoff W, van Veelen PA, Welling GW & Bruins AP 1994 'Characterization of a synthetic 37-residue fragment of a monoclonal antibody against herpes virus by capillary electrophoresis/electrospray (ionspray) mass spectrometry and252Cf plasma desorption mass spectrometry' Biological Mass Spectrometry 23, (6) 346-352 , DOI
Lasonder E, Schellekens GA & Welling GW 1994 'A fast and sensitive method for the evaluation of binding of phage clones selected from a surface displayed library' Nucleic Acids Res 22, (3) 545-546 Author Site
Lasonder E & Weringa WD 1990 'An NMR and DSC study of the interaction of phospholipid vesicles with some anti-inflammatory agents' Journal of Colloid and Interface Science 139, (2) 469-478 , DOI
Spronk CA, Jansen JF, Tessari M, Kaan AM, Aelen J, Lasonder E, Stunnenberg HG & Vuister GW 2001 'Sequence-specific assignment of the PAH2 domain of Sin3B free and bound to Mad1' J Biomol NMR 19, (4) 377-378 Author Site
Conference Papers
Marke R, Demkes M, Lasonder E, Hoogerbrugge PM, van Leeuwen FN & Scheijen B 2016 'P190BCR-ABL1 Signaling Modulates the Function of Tumour Suppressor Protein IKZF1' S26-S27 PEARL
Hanemann CO, Ferluga S, Dunn J, Bassiri K, Sharma V & Lasonder E 2016 'ANALYSIS OF THE PROTEOM AND PHOSHOPROTEOM OF MENINGIOMAS' 68-68
Ferluga S, Dunn J, Lasonder E, Sharma V, Hilton DA, Adams C & Hanemann CO 2016 'Proteome and phosphoproteome analysis of stratified meningiomas' S177-S177 PEARL
Hanemann CO, Zhou L, Ammoun S, Lasonder E, Sharma V, Muller J & Ercolano E 2015 'Abstract 704: Scaffold protein KSR1 is negatively regulated by merlin and promotes tumor development in merlin deficient tumors' 704-704 , DOI
Pradel G, Simon N, Lasonder E, Scheuermayer M, Kuehn A, Fischer R, Zipfel P & Skerka C 2013 'The impact of complement evasion on transmission of malaria' 243-243 , DOI
Mair GR, Lasonder E, Braks JAM, Garver LS, Wiegant JCAG, Dirks RW, Ponzi M, Pace T, Khan SM 2008 'Characterisation and functional analysis of a generally conserved Plasmodium berghei gametocyte translational repressor complex' S21-S21
Olivieri A, Silvestrini F, Lasonder E, Camarda G, Bertuccini L, Sanchez M, vande Vegte-Bolmer M, Luty AJF, Sauerwein R 2008 'Functional and proteornic analysis of the early gametocytes of Plasmodium falciparum' S75-S75
Khan S, van Dijk M, Franke-Fayard B, Lasonder E, Berry A, van Dooren M, Kocken C, Boehme U, Grainger M 2008 'Plasmodium ins and outs: merozoite proteomics and informed gene disruption' S84-S85