Dr Ansgar Poetsch
Profiles

Dr Ansgar Poetsch

Associate Professor/Reader in Proteomics

Plymouth University Peninsula Schools of Medicine and Dentistry

Role

Associate Professor in Proteomics

Qualifications

M.Sc. Bioinformatics, University of Manchester, 2005

Ph.D. Biochemistry, Technical University of Darmstadt, 1998

Dipl.-Ing. Chemical Technology, Technical University of Darmstadt, 1995 



Professional membership

German Society for Basic and Applied Microbiology

German Society for Proteome Research

Human Proteome Organisation (HUPO) 

Teaching interests

-omics technology, systems biology, biochemistry, biotechnology, microbiology


BHCS3001 Personal Research Project

Staff serving as external examiners

M.Sc. and Ph.D. program at CAS QiBEBT, Qingdao

Research interests

My research interest lies in the development and application of biological mass spectrometry to study membrane-related processes in living systems. Current research addresses three main areas:

1. Proteome turnover and membrane proteases
Membrane proteases have many important functional roles. Examples for microorganisms are quality control of membrane proteins and participation in quorum sensing. However, due to technical challenges, substrates and physiological role of membrane proteases remain largely elusive. In order to close this gap of knowledge, I a recently developed proteome turnover approach, employing metabolic labeling for the dissection of protein synthesis and degradation and data analysis using the web platform QuPE, to adequately address this challenge. Currently, the research foci are prokaryotic/microbial membrane proteases, LonB from the archaeon Haloferax volcanii and FtsH from the bacterium Corynebacterium glutamicum. The strategy is to use proteome turnover analysis in different strains and using different growth conditions to discover substrates based on changing proteolysis kinetics.


2. Sperm signal transduction
Mature sperm do not show transcription or translation. Therefore, proteomics is the favored method for global analysis of these cells. Especially, I am interested in changes in the proteome, including protein posttranslational modifications (PTMs) that are central to chemosensation and signal transduction. Ultimate goal is a systems biology interpretation of signal transduction, which requires accurate quantification of cellular components. Achieving this goal for integral membrane proteins poses additional challenges, such as selecting of an adequate standard for protein quantification. Here, I am active in developing methods to monitor temporal changes in PTMs and to elucidate subunit stoichiometries of membrane protein complexes. Understanding sensory signaling in mammalian sperm is key for the development of better diagnostic tools for male infertility and its treatment. This project is done in collaboration with U. Benjamin Kaupp (research center Caesar, Bonn) and Timo Strünker (University Hospital, Münster).

3. Regulation of lipid metabolism in Nannochloropsis
Harnessing the potential of algae for bioenergy sources, like the oil and the biohydrogen production, is highly attractive, but it still requires substantial research activities to become industrially feasible. Essentially, it is my aim for strain engineering to deepen the knowledge of molecular networks that can be manipulated to achieve desired traits (e.g. CO2 conversion rate, tailored lipid production) and to ultimately produce such strains by applying these techniques and knowledge. This aim requires a solid understanding of the molecular networks relevant for each goal. Relating to the changes of the proteome under different growth conditions and the role of protein posttranslational modifications in regulation of networks and enzyme activities, close to nothing is known. In collaboration with Jian Xu and other researchers from QiBEBT –omics technologies are employed on multiple levels (genomics, transcriptomics, proteomics and metabolomics) in combination with bioinformatics to untangle the networks and to integrate and link the obtained information in a database, which will become an invaluable resource for future research. 

Other research

I have been involved in industrial research projects with Qiagen, Evonik, Cube Biotech, and Merck

Research degrees awarded to supervised students

I have supervised 6 PhD students (currently supervising 3 PhD students);  16 MSc students.

Grants & contracts

Recent grants:


02/15-03/18  ERASysAPP research network „SysMetEx, Systems Biology of acidophile biofilms for efficient metal extraction” (Funding amount ~450.000€) 
 06/14-08/17  WTZ German/Argentinian collaboration project with Prof. de Castro, University Mar del Plata: “ProMikro, The role of membrane proteases for the adaptation of microorganisms to the environment“ (Funding amount ~27.000€)
 05/13-06/17  DFG/CDZ German/Chinese collaboration project with Prof. Kang Ning, QIBEBT-CAS, Qingdao: „Combination of single-cell sorting and proteomics to understand microbial heterogeneity“ (Funding amount: ~180.000€)
 01/10-06/13  BMBF research network: “GerontoMitoSys - Mitochondrial networks of pathways in ageing and life span control- a systems biology approach” (Funding amount: 180.000€), Project: “Quantitative analysis of age-related protein modifications”
 10/09-12/12  BMBF research network: ‘FlexFit – Improving the flexibility and fitness of Corynebacterium glutamicum for industrial biotechnology’ (Funding amount: 290.000€), Project: “Analysis of protein damage in C. glutamicum and its role for physiological fitness”
 10/09  Junior group funding of Ruhr University Bochum (Funding amount: ~30.000€)
 09/09-12/13  BMBF grant: ‘MetaZyme’ – a new approach for the kinetic analysis of metabolic pathways (focus on sphingolipid biosynthesis in yeast) (Funding amount: 1.550.000€)


Key publications are highlighted

Journals
Articles
Harst A, Albaum SP, Bojarzyn T, Trötschel C & Poetsch A 2017 'Proteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations' Journal of Proteomics 160, 1-7 , DOI PEARL
Ramallo Guevara C, Philipp O, Hamann A, Werner A, Osiewacz HD, Rexroth S, Rögner M & Poetsch A 2016 'Global Protein Oxidation Profiling Suggests Efficient Mitochondrial Proteome Homeostasis During Aging' Molecular & Cellular Proteomics 15, (5) 1692-1709 , DOI
Kaur H, Takefuji M, Ngai CY, Carvalho J, Bayer J, Wietelmann A, Poetsch A, Hoelper S, Conway SJ & Möllmann H 2016 'Targeted Ablation of Periostin-Expressing Activated Fibroblasts Prevents Adverse Cardiac Remodeling in MiceNovelty and Significance' Circulation Research 118, (12) 1906-1917 , DOI
Fechner S, Alvarez L, Bönigk W, Müller A, Berger TK, Pascal R, Trötschel C, Poetsch A, Stölting G & Siegfried KR 2015 'A K + -selective CNG channel orchestrates Ca 2+ signalling in zebrafish sperm' eLife 4, , DOI
Raju D, Schonauer S, Hamzeh H, Flynn KC, Bradke F, vom Dorp K, Dörmann P, Yildiz Y, Trötschel C & Poetsch A 2015 'Accumulation of Glucosylceramide in the Absence of the Beta-Glucosidase GBA2 Alters Cytoskeletal Dynamics' PLOS Genetics 11, (3) e1005063-e1005063 , DOI
Trötschel C & Poetsch A 2015 'Current approaches and challenges in targeted absolute quantification of membrane proteins' PROTEOMICS 15, (5-6) 915-929 , DOI
Cerletti M, Paggi RA, Ramallo Guevara C, Poetsch A & De Castro RE 2015 'Data in support of global role of the membrane protease LonB in Archaea : Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii' Data in Brief 4, 50-53 , DOI
Lange K, Poetsch A, Schmid A & Julsing MK 2015 'Enrichment and identification of Δ 9 -Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants' Data in Brief 4, 641-649 , DOI
Cerletti M, Paggi RA, Guevara CR, Poetsch A & De Castro RE 2015 'Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii' Journal of Proteomics 121, 1-14 , DOI
Seifert R, Flick M, Bonigk W, Alvarez L, Trotschel C, Poetsch A, Muller A, Goodwin N, Pelzer P & Kashikar ND 2015 'The CatSper channel controls chemosensation in sea urchin sperm' The EMBO Journal 34, (3) 379-392 , DOI
Bellenberg S, Díaz M, Noël N, Sand W, Poetsch A, Guiliani N & Vera M 2014 'Biofilm formation, communication and interactions of leaching bacteria during colonization of pyrite and sulfur surfaces' Research in Microbiology 165, (9) 773-781 , DOI
Pichlo M, Bungert-Plümke S, Weyand I, Seifert R, Bönigk W, Strünker T, Kashikar ND, Goodwin N, Müller A & Körschen HG 2014 'High density and ligand affinity confer ultrasensitive signal detection by a guanylyl cyclase chemoreceptor' The Journal of Cell Biology 207, (5) 675-675 , DOI
Pichlo M, Bungert-Plümke S, Weyand I, Seifert R, Bönigk W, Strünker T, Kashikar ND, Goodwin N, Müller A & Körschen HG 2014 'High density and ligand affinity confer ultrasensitive signal detection by a guanylyl cyclase chemoreceptor' The Journal of Cell Biology 206, (4) 541-557 , DOI
Plohnke N, Hamann A, Poetsch A, Osiewacz HD, Rögner M & Rexroth S 2014 'Proteomic analysis of mitochondria from senescent Podospora anserina casts new light on ROS dependent aging mechanisms' Experimental Gerontology 56, 13-25 , DOI
Guo X, Jiang C, Luo Y, Zhang M, Poetsch A & Liu S 2014 'Proteomic and molecular investigations revealed that Acidithiobacillus caldus adopts multiple strategies for adaptation to NaCl stress' Chinese Science Bulletin 59, (3) 301-309 , DOI
Jiang C-Y, Liu L-J, Guo X, You X-Y, Liu S-J & Poetsch A 2014 'Resolution of carbon metabolism and sulfur-oxidation pathways of Metallosphaera cuprina Ar-4 via comparative proteomics' Journal of Proteomics 109, 276-289 , DOI
Brenker C, Zhou Y, Müller A, Echeverry FA, Trötschel C, Poetsch A, Xia X-M, Bönigk W, Lingle CJ & Kaupp UB 2014 'The Ca 2+ -activated K + current of human sperm is mediated by Slo3' eLife 3, , DOI
Hennen E, Safina D, Haussmann U, Wörsdörfer P, Edenhofer F, Poetsch A & Faissner A 2013 'A LewisX Glycoprotein Screen Identifies the Low Density Lipoprotein Receptor-related Protein 1 (LRP1) as a Modulator of Oligodendrogenesis in Mice' Journal of Biological Chemistry 288, (23) 16538-16545 , DOI
Wolff D, ter Veld F, Köhler T & Poetsch A 2013 'Combined application of targeted and untargeted proteomics identifies distinct metabolic alterations in the tetraacetylphytosphingosine (TAPS) producing yeast Wickerhamomyces ciferrii' Journal of Proteomics 82, 274-287 , DOI
Vera M, Janosch C, Bellenberg S, Krok B, Sand W & Poetsch A 2013 'New Insights into the Biofilm Lifestyle and Metabolism of <i>Acidithiobacillus</i> Species from Analysis of High Throughput Proteomic Data' Advanced Materials Research 825, 111-114 , DOI
Haußmann U, Wolters DA, Fränzel B, Eltis LD & Poetsch A 2013 'Physiological Adaptation of the Rhodococcus jostii RHA1 Membrane Proteome to Steroids as Growth Substrates' Journal of Proteome Research 12, (3) 1188-1198 , DOI
ter Veld F, Wolff D, Schorsch C, Köhler T, Boles E & Poetsch A 2013 'Production of tetraacetyl phytosphingosine (TAPS) in Wickerhamomyces ciferrii is catalyzed by acetyltransferases Sli1p and Atf2p' Applied Microbiology and Biotechnology 97, (19) 8537-8546 , DOI
Trötschel C, Albaum SP & Poetsch A 2013 'Proteome turnover in bacteria: current status for Corynebacterium glutamicum and related bacteria' Microbial Biotechnology n/a-n/a , DOI
Vera M, Krok B, Bellenberg S, Sand W & Poetsch A 2013 'Shotgun proteomics study of early biofilm formation process of Acidithiobacillus ferrooxidans ATCC 23270 on pyrite' PROTEOMICS 13, (7) 1133-1144 , DOI
Haußmann U & Poetsch A 2012 'Global proteome survey of protocatechuate- and glucose-grown Corynebacterium glutamicum reveals multiple physiological differences' Journal of Proteomics 75, (9) 2649-2659 , DOI
Trötschel C, Albaum SP, Wolff D, Schröder S, Goesmann A, Nattkemper TW & Poetsch A 2012 'Protein Turnover Quantification in a Multilabeling Approach: From Data Calculation to Evaluation' Molecular & Cellular Proteomics 11, (8) 512-526 , DOI
Rexroth S, Poetsch A, Rögner M, Hamann A, Werner A, Osiewacz HD, Schäfer ER, Seelert H & Dencher NA 2012 'Reactive oxygen species target specific tryptophan site in the mitochondrial ATP synthase' Biochimica et Biophysica Acta (BBA) - Bioenergetics 1817, (2) 381-387 , DOI
Albaum SP, Hahne H, Otto A, Haußmann U, Becher D, Poetsch A, Goesmann A & Nattkemper TW 2011 'A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study' Proteome Science 9, (1) 30-30 , DOI
Poetsch A, Haußmann U & Burkovski A 2011 'Proteomics of corynebacteria: From biotechnology workhorses to pathogens' PROTEOMICS 11, (15) 3244-3255 , DOI
You X-Y, Guo X, Zheng H-J, Zhang M-J, Liu L-J, Zhu Y-Q, Zhu B, Wang S-Y, Zhao G-P & Poetsch A 2011 'Unraveling the Acidithiobacillus caldus complete genome and its central metabolisms for carbon assimilation' Journal of Genetics and Genomics 38, (6) 243-252 , DOI
Fränzel B, Trötschel C, Rückert C, Kalinowski J, Poetsch A & Wolters DA 2010 'Adaptation of Corynebacterium glutamicum to salt-stress conditions' PROTEOMICS 10, (3) 445-457 , DOI
Fränzel B, Poetsch A, Trötschel C, Persicke M, Kalinowski J & Wolters DA 2010 'Quantitative proteomic overview on the Corynebacterium glutamicum l-lysine producing strain DM1730' Journal of Proteomics 73, (12) 2336-2353 , DOI
Rietschel B, Arrey TN, Meyer B, Bornemann S, Schuerken M, Karas M & Poetsch A 2009 'Elastase Digests' Molecular & Cellular Proteomics 8, (5) 1029-1043 , DOI
Seelert H, Dani DN, Dante S, Hauß T, Krause F, Schäfer E, Frenzel M, Poetsch A, Rexroth S & Schwaßmann HJ 2009 'From protons to OXPHOS supercomplexes and Alzheimer's disease: Structure–dynamics–function relationships of energy-transducing membranes' Biochimica et Biophysica Acta (BBA) - Bioenergetics 1787, (6) 657-671 , DOI
Follmann M, Ochrombel I, Krämer R, Trötschel C, Poetsch A, Rückert C, Hüser A, Persicke M, Seiferling D & Kalinowski J 2009 'Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis' BMC Genomics 10, (1) 621-621 , DOI
Haußmann U, Qi S-W, Wolters D, Rögner M, Liu S-J & Poetsch A 2009 'Physiological adaptation of Corynebacterium glutamicum to benzoate as alternative carbon source - a membrane proteome-centric view' PROTEOMICS 9, (14) 3635-3651 , DOI
Fränzel B, Fischer F, Trötschel C, Poetsch A & Wolters D 2009 'The two-phase partitioning system - a powerful technique to purify integral membrane proteins of Corynebacterium glutamicum for quantitative shotgun analysis' PROTEOMICS 9, (8) 2263-2272 , DOI
Poetsch A & Wolters D 2008 'Bacterial membrane proteomics' PROTEOMICS 8, (19) 4100-4122 , DOI
Flaiz C, Utermark T, Parkinson DB, Poetsch A & Hanemann CO 2008 'Impaired intercellular adhesion and immature adherens junctions in merlin-deficient human primary schwannoma cells' Glia 56, (5) 506-515 , DOI
Poetsch A 2008 'Improved identification of membrane proteins by MALDI-TOF MS/MS using vacuum sublimated matrix spots on an ultraphobic chip surface' Journal of Biomolecular Techniques 19, (2) 129-138
Suhai T, Dencher NA, Poetsch A & Seelert H 2008 'Remarkable stability of the proton translocating F1FO-ATP synthase from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1' Biochimica et Biophysica Acta (BBA) - Biomembranes 1778, (4) 1131-1140 , DOI
Barriuso-Iglesias M, Schluesener D, Barreiro C, Poetsch A & Martín JF 2008 'Response of the cytoplasmic and membrane proteome of Corynebacterium glutamicum ATCC 13032 to pH changes' BMC Microbiology 8, (1) 225-225 , DOI
Luedke A, Kraemer R, Burkovski A, Schluesener D & Poetsch A 2007 'A proteomic study of Corynebacterium glutamicum AAA plus protease FtsH' BMC MICROBIOLOGY 7, Author Site , DOI
Polen T, Schluesener D, Poetsch A, Bott M & Wendisch VF 2007 'Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis' FEMS Microbiology Letters 273, (1) 109-119 , DOI
Qi S-W, Chaudhry MT, Zhang Y, Meng B, Huang Y, Zhao K-X, Poetsch A, Jiang C-Y, Liu S & Liu S-J 2007 'Comparative proteomes of grown on aromatic compounds revealed novel proteins involved in aromatic degradation and a clear link between aromatic catabolism and gluconeogenesis fructose-1,6-bisphosphatase' PROTEOMICS 7, (20) 3775-3787 , DOI
Schluesener D, Rögner M & Poetsch A 2007 'Evaluation of two proteomics technologies used to screen the membrane proteomes of wild-type Corynebacterium glutamicum and an L-lysine-producing strain' Analytical and Bioanalytical Chemistry 389, (4) 1055-1064 , DOI
Chaudhry MT, Huang Y, Shen X-H, Poetsch A, Jiang C-Y & Liu S-J 2007 'Genome-wide investigation of aromatic acid transporters in Corynebacterium glutamicum' Microbiology 153, (3) 857-865 , DOI
Gertz M, Seelert H, Dencher NA & Poetsch A 2007 'Interactions of rotor subunits in the chloroplast ATP synthase modulated by nucleotides and by Mg2+' Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1774, (5) 566-574 , DOI
Poetsch A, Berzborn RJ, Heberle J, Link TA, Dencher NA & Seelert H 2006 'Biophysics and Bioinformatics Reveal Structural Differences of the Two Peripheral Stalk Subunits in Chloroplast ATP Synthase' Journal of Biochemistry 141, (3) 411-420 , DOI
Fischer F & Poetsch A 2006 'Protein cleavage strategies for an improved analysis of the membrane proteome' PROTEOME SCIENCE 4, Author Site , DOI
Fischer F, Wolters D, Rögner M & Poetsch A 2006 'Toward the Complete Membrane Proteome' Molecular & Cellular Proteomics 5, (3) 444-453 , DOI
Schluesener D, Fischer F, Kruip J, Rögner M & Poetsch A 2005 'Mapping the membrane proteome of' PROTEOMICS 5, (5) 1317-1330 , DOI
Poetsch A, Rexroth S, Heberle J, Link TA, Dencher NA & Seelert H 2003 'Characterisation of subunit III and its oligomer from spinach chloroplast ATP synthase' Biochimica et Biophysica Acta (BBA) - Biomembranes 1618, (1) 59-66 , DOI
Poetsch A, Molday LL & Molday RS 2001 'The cGMP-gated Channel and Related Glutamic Acid-rich Proteins Interact with Peripherin-2 at the Rim Region of Rod Photoreceptor Disc Membranes' Journal of Biological Chemistry 276, (51) 48009-48016 , DOI
Poetsch A, Neff D, Seelert H, Schägger H & Dencher NA 2000 'Dye removal, catalytic activity and 2D crystallization of chloroplast H+-ATP synthase purified by blue native electrophoresis' Biochimica et Biophysica Acta (BBA) - Biomembranes 1466, (1-2) 339-349 , DOI
SEELERT H, POETSCH A, ROHLFS M & DENCHER NA 2000 'Dye-ligand chromatographic purification of intact multisubunit membrane protein complexes: application to the chloroplast H+-FoF1-ATP synthase' Biochemical Journal 346, (1) 41-41 , DOI
Poetsch A 2000 'Structural biology: Proton-powered turbine of a plant motor' Nature 418-419 , DOI
Poetsch A, Seelert H, Meyer zu Tittingdorf J & Dencher NA 1999 'Detergent Effect on Anion Exchange Perfusion Chromatography and Gel Filtration of Intact Chloroplast H+-ATP Synthase' Biochemical and Biophysical Research Communications 265, (2) 520-524 , DOI
Conference Papers
Safina D, Hennen E, Huassmann U, Woersdoerfer P, Edenhofer F, Poetsch A & Faissner A 2013 'LOW DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 1 (LRP1) IS EXPRESSED ON RADIAL GLIA CELLS AND CONTROLS THEIR DIFFERENTIATION TOWARDS OLIGODENDROGLIA' S53-S53
Poetsch A, Wolff D, ter Veld F & Koehler T 2013 'The tetraacetyl phytosphingosine (TAPS) biosynthesis network of Wickerhamomyces ciferrii' 190-190

Other academic activities

Adjunct Professor Qingdao Institute for Bioenergy & Biotechnology (QiBEBT)